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单纯疱疹病毒1型US11蛋白结合DNA甲基转移酶3A的作用研究 预览

Study on US11 protein binding DNA methyltransferase 3A of herpes simplex virus type 1
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摘要 目的通过免疫共沉淀联合质谱分析筛查与单纯疱疹病毒1型(HSV-1)中US11蛋白存在潜在相互作用的宿主蛋白。方法使用荧光标记的HSV-1病毒感染人成纤维细胞(HFF),通过免疫共沉淀(IP)方法对US11及其相互作用蛋白进行提取和纯化,并与质谱(MS)联用来鉴定相关蛋白。结果利用IP-MS分析技术成功筛选出7个与US11存在潜在相互作用宿主蛋白,其中DNA甲基转移酶3A(DNMT3A)被定性为一种新的US11结合蛋白。结论HSV-1病毒US11蛋白与哺乳动物DNA甲基转移酶机制的功能关联,DNMT3A是HSV-1感染所需的有效宿主因子。 Objective To screen host proteins potentially interacting with US11 protein in herpes simplex virus type 1(HSV-1)by immunoprecipitation combined with mass spectrometry.Methods The HFF was infected with the fluorescently labeled HSV-1 virus,and US11 and its interacting protein was extracted and purified by co-immunoprecipitation,and mass spectrometry was performed to identify the related protein.Result IP-MS analysis technology was used to successfully screen 7 host protein with potential interaction with US11,DNA methyltransferases 3A was identified as a new US11 binding protein.Conclusion Our results highlight a functional association of virus US11 protein with the mammalian DNA methyltransferase machinery,indicating DNMT3A as a host factor required for effective HSV-1 infection.
作者 钟霞 潘建华 童坚 ZHONG Xia;PAN Jianhua;TONG Jian(Haikou People′s Hospital,Department of Clinical Laboratory,Haikou 570208,China)
出处 《实用医学杂志》 CAS 北大核心 2019年第1期56-60,共5页 The Journal of Practical Medicine
关键词 免疫共沉淀 质谱 单纯疱疹病毒1型 US11蛋白 DNA甲基转移酶3A co-immunoprecipitation mass spectrometry herpes simplex virus type 1(HSV-1) US11 protein DNA methyltransferases 3A(DNMT3A)
作者简介 通信作者:钟霞,E-mail:lks734@163.com.
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