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颈腰康胶囊的抗炎消肿药效物质基础研究 预览

Study on Anti-inflammatory and Detumescent Pharmacodynamic Material Basis of Jingyaokang Capsule
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摘要 目的:研究颈腰康胶囊抗炎消肿药效的物质基础,为该制剂的二次开发、质量控制方法建立和技术改造升级提供依据。方法:采用不同溶剂以及大孔吸附树脂法对颈腰康胶囊进行组分提取分离,获得A组分(总体富集部位)、B组分(氯仿萃取部位)、C组分(水层部位)和D组分(60%乙醇洗脱部位)。通过小鼠耳廓肿胀实验和大鼠足跖肿胀实验,以醋酸地塞米松为阳性对照药物,考察颈腰康胶囊及其不同提取部位(A、B、C、D组分)的抗炎消肿作用,筛选功效部位。采用超高效液相色谱-四极杆飞行时间质谱技术(UPLC-Q-TOF-MS)解析功效部位,鉴定其中所含化合物并进行组方药材归属。结果:B组分(氯仿萃取部位)+D组分(60%乙醇洗脱部位)对大鼠或小鼠的抗炎消肿疗效与颈腰康胶囊相当,提示二者发挥了协同抗炎消肿作用,是颈腰康胶囊的功效部位。UPLC-Q-TOF-MS检测及鉴定结果显示,B组分中含有士的宁、黄柏碱、杠柳苷元、石松四酮醇、11-羰基-β-乳香酸等13个化合物,分别归属于马钱子、防己、香加皮、伸筋草、乳香等药材;D组分中含有腺嘌呤、羟基红花黄色素A、黄柏碱、Neoeriocitrin、姜状三七皂苷R1等7个化合物,分别归属于地龙、红花、防己、骨碎补、牛膝等药材。结论:颈腰康胶囊具有显著的抗炎消肿作用,其氯仿萃取部位与60%乙醇洗脱部位可能共同为其抗炎消肿的功效部位,所含主要成分为生物碱类、黄酮类及乳香酸类化合物。 OBJECTIVE:To study the anti-inflammatory and detumescent pharmacodynamic material basis of Jingyaokang capsule,and to provide reference for secondary development,the establishment of quality control method and technological upgrading of the preparations.METHODS:The constituents of Jingyaokang capsule were extracted and separated with different solvents and macroporous adsorption resin to obtain constituent A(overall enrichment part),constituent B(chloroform extraction part),constituent C(water-course part)and constituent D(elution part of 60%ethanol).Using dexamethasone acetate as positive control,the anti-inflammatory and detumescent effects of Jingyaokang capsule and different extraction parts(constituents A,B,C,D)were investigated by mice ear edema and rat paw edema tests to screen the active fraction.UPLC-Q-TOF-MS method was used to analyze active constituent,identify compounds and attribute medicinal material.RESULTS:Anti-inflammatory and detumescent effects of constituent B(chloroform extraction part)+constituent D(elution part of 60%ethanol)were similar to those of Jingyaokang capsule in rats or mice,indicating both had synergistic anti-inflammatory and detumescent effects and were active constituents of Jingyaokang capsule.UPLC-Q-TOF-MS detection and identification showed that constituent B contained 13 compounds as strychnine,phellodendrine,periplogenin,tetraketone alcohol,11-carbonyl-β-mastic acid,attributing to Strychnos nux-vomica,Stephania tetrandra,Periploca sepium,Lycopodium japonicum,Boswellia carterii,etc.Constituent D contained 7 compounds as adenine,hydroxysafflower yellow A,phellodendrine,neoeriocitrin,zingibroside R1,attributing to rainworm,Carthamus tinctorius,Stephania tetrandra,Davallia mariesii,Achyranthes bidentata,etc.CONCLUSIONS:Jingyaokang capsule shows the significant anti-inflammation and detumescent effects.The chloroform extraction part is synergistic with 60%ethanol elution part,which are the active constituents of anti-inflammation and detumescence,mainly including alkaloids,fl
作者 徐杰 姜文月 王雅俪 王美慧 魏晓雨 边雨 XU Jie;JIANG Wenyue;WANG Yali;WANG Meihui;WEI Xiaoyu;BIAN Yu(Jilin Modern Chinese Medicine Engineering and Research Center Co.,Ltd.,Changchun 130012,China;Xiuzheng Pharmaceutical Group Incorporated Co.,Ltd.,Jilin Tonghua 134001,China)
出处 《中国药房》 CAS 北大核心 2019年第4期478-483,共6页 China Pharmacy
基金 吉林省科技发展计划项目(No.YYZX201508).
关键词 颈腰康胶囊 提取 分离 抗炎 消肿 超高效液相色谱-四极杆飞行时间质谱技术 活性成分 物质基础 Jingyaokang capsule Extraction Separation Anti-inflammation Detumescence UPLC-Q-TOF-MS Active constituent Material basis
作者简介 徐杰:助理工程师,药师,硕士。研究方向:中药新药及大品种二次开发。电话:0431-89255164。E-mail:xu908613808@163.com;通信作者:姜文月,工程师,博士。研究方向:中药新药及大品种二次开发。电话:0431-89255164。E-mail:jiangwenyue1987@126.com.
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