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Mansonone F对人宫颈癌细胞增殖、凋亡、侵袭及迁移的影响 预览

Effects of Mansonone F on proliferation,apoptosis,invasion,and migration of cervical cancer Hela cells
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摘要 目的观察Mansonone F对人宫颈癌Hela细胞体外增殖、凋亡、侵袭和迁移能力的影响,并探讨其作用机制。方法取对数生长期的Hela细胞,分别加入2.5、5、10μmol/L Mansonone F培养。采用MTT法测算细胞增殖抑制率,流式细胞术检测细胞凋亡率,Transwell小室实验观察细胞侵袭能力和细胞迁移能力,RT-PCR法检测细胞黏着斑激酶(FAK)mRNA表达,Western blotting法检测FAK、磷酸化黏着斑激酶(p-FAK)蛋白表达。结果Mansonone F作用Hela细胞24、48、72 h后,随着药物浓度的增加和作用时间的延长,细胞增殖抑制率均升高(P均<0.05)。随着药物浓度的增加,细胞早期凋亡率、晚期凋亡率均逐渐升高,10μmol/L浓度下的细胞凋亡率高于其他各浓度(P均<0.01)。随着药物浓度的增加,侵袭实验和迁移实验的穿膜细胞数目逐渐减少,10μmol/L浓度下的穿膜细胞数最少(P<0.01);以2.5、5、10μmol/L Mansonone F作用Hela细胞24 h后,随着药物浓度的升高,细胞FAK mRNA及蛋白、p-FAK蛋白表达均降低,10μmol/L作用下的细胞FAK mRNA及蛋白、p-FAK蛋白表达最低(P均<0.01)。结论Mansonone F可显著抑制Hela细胞的增殖、诱导细胞凋亡,抑制细胞侵袭和迁移,其机制可能与抑制FAK表达、下调FAK的磷酸化水平有关。 Objective To investigate the effects of Mansonone F on proliferation,apoptosis,invasion,and migration of human cervical cancer Hela cells and to explore the possible mechanism.Methods Hela cells in the logarithmic phase were selected and treated with 2.5,5,and 10μM Mansonone F for 24 h,respectively.The effects of Mansonone F on cell viability,apoptosis rate,invasion and migration abilities of Hela cells were detected by MTT assay,flow cytometry and Transwell assay,respectively.RT-PCR and Western blotting were used to detect the expression of focal adhesion kinase(FAK)mRNA,and the levels of FAK and phosphorylated FAK proteins.Results Mansonone F inhibited the proliferation of Hela cells at 24,48,and 72 h,in a dose-dependent manner(P<0.05).With the increase of drug concentration,the early and late apoptotic rates of Hela cells increased gradually,and the apoptotic rate at 10μmol/L was higher than that at other concentrations(all P<0.01).With the increase of drug concentration,the cell invasion and migration were markedly suppressed,and the number of penetrating cells was the lowest at 10μmol/L(P<0.01).Furthermore,Mansonone F significantly down-regulated the mRNA and protein expression of FAK,and the level of phosphorylated FAK protein after Hela cells were treated with 2.5,5,and 10μmol/L Mansonone F for 24 h.The lowest protein expression was observed at 10μmol/L(P<0.01).Conclusion Mansonone F inhibits the proliferation,invasion,and migration,and induces the apoptosis of Hela cells by inhibiting the FAK expression,and down-regulating the level of phosphorylated FAK.
作者 晁晶 齐翌婷 CHAO Jing;QI Yiting(The Fifth Affiliated Hospital of Zunyi Medical University,Zhuhai 519090,China)
出处 《山东医药》 CAS 2019年第5期33-36,共4页 Shandong Medical Journal
基金 广东省珠海市卫计局医学科研基金资助项目(201732)。
关键词 宫颈癌 MansononeF HELA细胞 细胞增殖 细胞凋亡 细胞侵袭 细胞迁移 黏着斑激酶 cervical carcinoma Mansonone F Hela cells cell proliferation apoptosis cell invasion cell migration focal adhesion kinase
作者简介 第一作者:晁晶(1983-),女,主治医生,主要研究方向为妇科肿瘤的诊治。E-mail:67949136@qq.com.
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