期刊文献+

大规模平行测序在混合斑检测分析中的应用前景 预览

Application Prospect of Massively Parallel Sequencing in Mixed Stain Detection
在线阅读 下载PDF
收藏 分享 导出
摘要 混合斑是性犯罪案件中常见的生物检材,对其分析及结果解释是法医学检验中的难点之一。目前用于混合斑检测的遗传标记主要依赖于毛细管电泳(capillary electrophoresis,CE)分离技术,而检测结果分析方法主要为包含率法及似然率法。由于CE技术分辨率有限,并不能充分发挥各遗传标记的效能,因此拆分混合斑的能力有限。近年来,兴起的大规模平行测序(massively parallel sequencing,MPS)技术不仅能获得遗传标记的碱基序列信息,而且具有并行检测多种遗传标记的能力,衍生出新的分析方法,为法医混合斑检测分析带来了新的契机。本文拟对混合斑分析常规技术及大规模平行测序技术在混合斑中的应用前景进行综述,旨在为后期研究及实践提供参考和借鉴。 Mixed stains is the common biological sample in sexual crime cases. Its analysis and DNA profiles interpretation are one of the difficulties in forensic examination. The current genetic marking of mixed stain detection mainly rely on capillary electrophoresis (CE) separation technology, and the analysis methods of the results are mainly inclusion rate and likelihood methods. Because CE has limited resolution and is not able to exploit the efficacy of each genetic marker, its ability to split mixed stain is limited. In recent years, the emerging massively parallel sequencing (MPS) technique not only can obtain the base sequence information of genetic markers, but also is capable of detecting multiple genetic markers simultaneously, and thus derives new analytical methods, bringing new opportunities for forensic detection and analysis of mixed stain. This paper intends to review the application prospects of conventional mixed stain analyses and MPS technique, therefore to provide references for later research and practice.
作者 刘京 何光林 侯一平 王正 LIU Jing;HE Guang-lin;HOU Yi-ping;WANG Zheng(Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan Uni?versity, Chengdu 610041, China)
出处 《法医学杂志》 CAS CSCD 2019年第1期84-88,94共6页 Journal of Forensic Medicine
基金 国家自然科学青年基金资助项目(81501635).
关键词 法医遗传学 混合斑 大规模平行测序 二代测序 综述 forensic genetics mixed stain massively parallel sequencing second generation sequencing review
作者简介 刘京(1993—),女,博士研究生,主要从事法医遗传学研究;E-mail:1026911516@qq.com;通信作者:王正,男,副研究员,主要从事法医遗传学研究;E-mail:wangzhengtim@scu.edu.cn.
  • 相关文献

参考文献1

二级参考文献45

  • 1Parson W, Parsons TJ, Scheithauer R, et al. Popu- lation data for 101 Austrian Caucasian mitochondrial DNA d-loop sequences: application of mtDNA se- quence analysis to a forensic case[J]. Int J Legal Med, 1998,111 (3) : 124-132. 被引量:1
  • 2Schneider PM, Seo Y, Rittner C. Forensic mtDNA hair analysis excludes a dog from having caused a traffic accident [J]. Int J Legal Med, 1999,112 (5) : 315- 316. 被引量:1
  • 3Bender K, Schneider PM, Rittner C. Application of mtDNA sequence analysis in forensic casework for the identification of human remains[J]. Forensic Sci Int,2000,113(1-3) : 103-107. 被引量:1
  • 4Laber TL, Giese SA, Iverson JT, et al. Validation studies on the forensic analysis of restriction frag- ment length polymorphism (RFLP) on LE agarose gels without ethidium bromide: effects of contaminants, sunlight, and the electrophoresis of varying quantities of deoxyribonucleic acid (DNA)[J]. J Forensic Sci, 1994,39(3) :707-730. 被引量:1
  • 5Budowle B, Baechtel FS, Comey CT, et al. Simple protocols for typing forensic biological evidence: chemiluminescent detection for human DNA quantita- tion and restriction fragment length polymorphism (RFLP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms [J]. Electrophoresis, 1995,16(9) :1559-1567. 被引量:1
  • 6Parson W, Diir A. EMPOP--a forensic mtDNA database[J]. Forensic Sci Int Genet,2007,1 (2) : 88-92. 被引量:1
  • 7Sobrino B, Carracedo A. SNP typing in forensic ge- netics: a review[J]. Methods Mol Biol,2005,297 : 107- 126. 被引量:1
  • 8Sobrino B, Bri6n M, Carracedo A. SNPs in forensic genetics: a review on SNP typing methodologies[J]. Forensic Sci Int,2005,154(2-3) : 181-194. 被引量:1
  • 9Lavebratt C, Sengul S. Single nucleotide polymorphism (SNP) allele frequency estimation in DNA pools us- ing Pyrosequencing[J]. Nat Protoc,2006,1 (6):2573- 2582. 被引量:1
  • 10B#rsting C, Morling N. Next generation sequencing and its applications in forensic genetics[J]. Forensic Sci Int Genet,2015,18:78-89. 被引量:1

共引文献3

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈