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干酪乳杆菌对虫草素的生物转化研究 预览

Study on Biotransformation of Cordycepin by Lactobacillus casei
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摘要 探讨干酪乳杆菌( Lactobacillus casei)对虫草素的生物转化。采用L. casei与虫草素共培养的方法,以虫草素水溶液为底物,接入L. casei 活菌体进行厌氧培养,培养液经高效液相色谱(HPLC)和液质联谱(LC/MS)检测后有新产物生成,标记为CA。产物CA由制备液相分离、纯化后经核磁共振波谱(NMR)检测,被确定为3′-脱氧肌苷(3′-Deoxyinosine)。结果表明, L. casei 能够对虫草素进行生物转化,可将虫草素C-6位的氨基水解为羟基,生成3′-脱氧肌苷,并在培养72 h时使虫草素转化率达91.2%。 Biotransformation of cordycepin by Lactobacillus casei was investigated. L. casei was incubated anaerobically and cultured with cordycepin at 37 ℃ for 72 h. A new product in the biotransformation solution, marked as CA, were detected by high performance liquid chromatography (HPLC) and liquid chromatograph/mass spectrometer (LC/MS). CA was then isolated and purified by preparative liquid chromatograph, and determined structurally by nuclear magnetic resonance (NMR) techniques. As a result, CA was identified as 3′-deoxyinosine. The result suggested that L. casei showed potent ability to transform cordycepin to 3′-deoxyinosine. Cordycepin was converted to 3′-deoxyinosine by L. casei , where amino group of cordycepin was hydrolyzed into hydroxyl group at C-6, and produced 3′-deoxyinosine. The conversion rate of cordycepin was 91.2% in 72 h.
作者 肖璇 杨伟超 杜蓓蓓 徐慧 赵临襄 XIAO Xuan;YANG Wei-chao;DU Bei-bei;XU Hui;ZHAO Lin-xiang(Coll. of Pharm. Engin.,Shenyang Pharm. Uni.,Benxi 117004;Key Lab. of Pollut′n Ecol. & Environm′l Engin.,Inst. of Appl. Ecol.,Chinese Acad. of Sci.,Shenyang 110016)
出处 《微生物学杂志》 CAS CSCD 2019年第1期32-36,共5页 Journal of Microbiology
基金 国家自然科学基金项目(30911120071).
关键词 干酪乳杆菌 虫草素 生物转化 3′-脱氧肌苷 Lactobacillus casei cordycepin biotransformation 3′-deoxyinosine
作者简介 肖璇 女,硕士研究生。研究方向为环境微生物。E-mail:646331279@qq.com;通讯作者,徐慧 男,博士,研究员,博士生导师。研究方向为环境微生物。E-mail:xuhui@iae.ac.cn;通讯作者,赵临襄 女,博士,教授,博士生导师。研究方向为药物化学。E-mail:linxiang.zhao@vip.sina.com.
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