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云芝与槐耳抗氧化活性评价及低极性组分GC——MS成分分析 预览

Antioxidant Activity Evaluation and Low-Polarity Components Analysis of Coriolus versicolor and Trametes robiniophila
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摘要 目的:评价两种多孔菌科药食真菌云芝(Coriolusversicolor)和槐耳(Trametesrobiniophila)多溶剂提取部位的抗氧化、抗菌活性,并对它们低极性部位进行GC-MS成分分析。方法:采用索氏提取法获得两种真菌的乙醇提取浸膏,依次用石油醚、乙酸乙酯、正丁醇和蒸馏水再浸提浸膏,得四种不同溶剂的提取产物。分别采用DPPH自由基清除能力,平板打孔法测定各组分的抗氧化和抗菌活性,最后使用GC-MS对两种真菌的石油醚萃取物进行成分分析。结果:两种真菌各溶剂提取物抗氧化活性呈现明确量效关系,云芝乙酸乙酯与正丁醇部位具有较高活性。两种真菌的各溶剂提取物均无明显的抑菌效果。GC-MS分析结果显示云芝和槐耳石油醚部分各检测到27和39种化合物,其中亚油酸甲酯(云芝23.92%,槐耳4.29%),邻苯二甲酸单乙基己基酯(云芝18.68%,槐耳9.60%),2-羟基-二十四酸甲酯(云芝15.78%,槐耳15.54%),棕榈酸甲酯(云芝3.17%,槐耳3.78%),麦角甾-14,22-二烯-3-醇(云芝3.79%,槐耳10.38%)等在两种真菌中含量较高。结论:此研究明确了两种多孔菌科药食真菌中的部分化学成分以及其在抗氧化和抗菌方面的效用,为其在抗衰老药物、保健药食方面的开发应用提供了一定的理论依据。 Objective: To evaluate the antioxidant and antibacterial activity of two kinds of polyporaceae mushrooms (Coriolus versicolor and Trametes robiniophila),and investigate chemical constituents of their low polarity part. Methods: Extracting the powder of two mushrooms by soxhlet method.The above extracts were sequentially extracted with petroleum ether,ethyl acetate,n-butanol and distilled water. The antioxidant and antibacterial activity of different solvent extracts were investigated by the DPPH free radical clearance method and agar diffusion plate method.Finally,the petroleum ether extracts of the two mushrooms were methylation and analyzed by GC-MS.Results: The antioxidant activity of each solvent extract of the two mushrooms showed clear dose-effect relationship,and the ethyl acetate and n-butanol extract from Coriolus versicolor had stronger antioxidant activity.However,there was no obvious antibacterial effect of these two mushrooms.The results of GC-MS analysis showed that there were 27 compounds in petroleum ether extraction part of the Coriolus versicolor,and 39 compounds in Trametes robiniophila.Methyl linoleate,1,2-benzenedicarboxylic acid,methyl 2-hydroxy-tetracosanoate,palmitic acid and ergosta-14,22-dien-3-ol,(3β,5α,22 E) had a high contents in two mushrooms.Conclusion: This study had clarified part of the chemical constituents of these two mushrooms,and their antibacterial and antioxidation activity had been studied.It would provide some theoretical basis for their further development and utilization in anti-aging drugs and health food.
作者 袁诗俊 贺新生 袁小红 黄毅 YUAN Shi-jun;HE Xin-sheng;YUAN Xiao-hong;HUANG Yi(School ol Life Science and Engineering,Southwest University of Science and Technology, Mianyang 621010, China)
出处 《食品工业科技》 CAS 北大核心 2019年第6期1-5,12共6页 Science and Technology of Food Industry
基金 国家自然科学基金(21272189) 西南科技大学博士基金(16zx7161) 国家级大学生创新训练计划项目(201710619023)。
关键词 气相色谱-质谱 抗氧化活性 抗菌活性 多孔菌科 药食真菌 gas chromatography-mass spectrometry(GC-MS) antioxidant activity antibacterial activity polyporaceae edible-medicinal mushroom
作者简介 袁诗俊(1998-),男,大学本科,研究方向:天然产物开发,E-mail:18681262879@163.com;通讯作者:黄毅(1979-),男,博士,讲师,研究方向:真菌天然产物开发,E-mail:huangyi@swusl.edu.cn。
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