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PTPN6基因对人食管鳞状细胞癌Eca109和Yes-2细胞恶性生物学行为的影响

Effects of PTPN6 on malignant biological behaviors of human esophageal squamous cancer Eca109 and Yes-2 cells
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摘要 目的:探讨非受体型蛋白酪氨酸磷酸酶6(non-receptor protein tyrosine phosphatase 6,PTPN6)基因在不同食管鳞状细胞癌细胞株中的表达及其对Eca109、Yes-2细胞恶性生物学行为的影响。方法:应用qPCR法检测不同食管鳞癌细胞株(TE1、Eca109、Kyse150、Kyse170、Yes-2)中PTPN6 mRNA的表达水平,以pcDNA3.1-PTPN6质粒分别瞬时转染食管鳞癌细胞株Eca109和Yes-2,应用qPCR和Wb法检测PTPN6 mRNA和蛋白的表达水平,并应用MTS、克隆形成实验、划痕实验和Transwell法检测过表达PTPN6基因对食管鳞癌细胞恶性生物学行为的影响。结果:PTPN6基因在5种食管鳞癌细胞侏中表达均明显下调(均P<0.05)。与转染空载体的对照组相比,经pcDNA3.1-PTPN6转染后,Eca109和Yes-2细胞均高水平表达PTPN6(P<0.05或P<0.01);过表达PTPN6基因后,Eca109和Yes-2细胞的增殖、迁移和侵袭能力均明显被抑制(均P<0.05)。结论:PTPN6基因高表达能抑制食管鳞癌细胞的增殖、迁移和侵袭,其可能是影响食管鳞癌细胞生物学特性的一个重要因素。 Objective:To detect the expression of non-receptor protein tyrosine phosphatase 6(PTPN6)in different esophageal squamous cell carcinoma(ESCC)cell lines,and to investigate its effect on proliferation,migration and invasion ability of Eca109 and Yes-2 cell lines.Methods:qPCR was applied to detect the mRNA expression of PTPN6 in different ESCC cell lines(TE1,Eca109,Kyse150,Kyse170 and Yes-2).pcDNA3.1-PTPN6 plasmid was transiently transfected into Eca109 and Yes-2 cells respectively.The expression of PTPN6 was detected by real-time PCR and Wb.The effects of PTPN6 over-expression on the biological behaviors of ESCC cells were detected by MTS,colony formation assay,wound healing assay and Transwell assay,respectively.Results:The mRNA expression of PTPN6 was remarkably reduced in ESCC cell lines(TE1,Eca109,Kyse150,Kyse170 and Yes-2)compared to normal esophageal epithelial cells(HEEpiC)(P<0.05).Compared to the mock cells,significant up-regulation of PTPN6 was detected in pcDNA3.1-PTPN6 transfected Eca109 and Yes-2 cells(all P<0.05 or P<0.01);PTPN6 over-expression led to a significant inhibition in migration and invasion ability of Eca109 and Yes-2 cells(all P<0.05).Conclusions:Over-expression of PTPN6 may inhibit the proliferation,migration and invasion of ESCC cells,which might be an important factor influencing the biological characteristics of ESCC cells.
作者 刘磊 杨柳 牛云峰 梁佳 沈素朋 LIU Lei;YANG Liu;NIU Yunfeng;LIANG Jia;SHEN Supeng(Department of Thoracic Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China;Cancer Institute,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2019年第3期273-279,共7页 Chinese Journal of Cancer Biotherapy
基金 国家自然科学基金资助项目(No.81572441) 河北省医学科学研究重点课题资助项目(No.20170700).
关键词 食管鳞状细胞癌 非受体型蛋白酪氨酸磷酸酶6基因 ECA109细胞 Yes-2细胞 增殖 迁移 侵袭 esophageal squamous cell carcinoma non-receptor protein tyrosine phosphatase 6(PTPN6)gene Eca109 cell Yes-2 cell proliferation migration invasion
作者简介 刘磊(1978-),男,博士,主治医师,主要从事上消化道肿瘤病因学的研究,E-mail:abchbmu525@163.com;通信作者:沈素朋(SHEN Supeng,corresponding author),博士,助理研究员,主要从事肿瘤病因学及肿瘤发生机制的研究,E-mail:shsyssp2012@163.com.
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