In order to explore the effect of freezing damage caused by spring cold spells on the transcription profiling of young shoots of different tea cultivars, transcriptome analysis of the frozen and unfrozen shoots was performed on tea cultivar ‘Longjing 43’ and ‘Zhongcha 126’ during the cold spells. A total of 1 012 and 1 079 differentially expressed genes (DEGs) were identified in two cultivars with 284 DEGs overlapped. Then, eighteen DEGs were selected for Real-time quantitative PCR verification. The results were consistent with the transcriptome sequencing results, indicating that the transcriptome data was reliable. Utilizing the GO and KEGG pathway databases, the enriched pathways of the DEGs were identified, which included photosynthesis, carbon metabolism and cytochrome P450. This indicates that the cold spells cause serious damage to the basal metabolism related to the normal growth and development of young tea shoots and inhibit the expression of related genes. Subsequently, the expression cluster analysis of 284 overlapped DEGs showed that 99 DEGs exhibited the opposite expression patterns in two cultivars. The involved biological processes included MAPK signaling pathway, glutathione and phenylpropanoid metabolism. It was speculated that these DEGs were related to different signal transduction patterns in ‘Longjing 43’ and ‘Zhongcha 126’ in response to low temperature stress.
Journal of Tea Science
differentially expressed genes