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焦炉工多环芳烃接触与外周血单个核细胞CYP1A1基因和酶活力的关系

Association of polycyclic aromatic hydrocarbon exposure and CYP1A1 gene and enzyme activity of peripheral blood monocyte cells in coke oven workers
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摘要 目的探讨焦炉工多环芳烃(PAHs)接触与外周血单个核细胞CYP1A1基因和酶活力的相关关系,为焦炉工健康损害的生物监测提供一定的基础。方法对118名焦炉工和63名对照人群(同一企业的能源动力工)进行问卷调查,用高效液相色谱-荧光检测器法检测工人班后尿中1-羟基芘(1-OH-Py)的浓度;采集晨起空腹肘静脉血,分离外周血单个核细胞(PBMC),用彗星实验检测DNA损伤情况;用实时荧光定量-聚合酶链反应(QT-PCR)法和荧光分光光度法检测外周血单个核细胞中CYP1A1基因表达和CYP1A1酶(EROD)活力;用单因素方差分析、多元线性回归等统计方法分析尿1-OH-Py与CYP1A1基因和酶活力之间的关系。结果与对照组比较,焦炉工尿1-OH-Py浓度和DNA尾距明显较高,外周血单个核细胞CYP1A1基因表达水平和EROD活力明显较低,差异均有统计学意义(P<0.05)。多元线性回归结果显示,尿中1-OH-Py浓度每增加10倍,焦炉工外周血单个核细胞CYP1A1基因表达水平降低0.77个单位(95%CI:-1.33~-0.21),EROD活力降低0.25个单位(95%CI:-0.67~-0.17)。结论焦炉工PAHs接触可引起外周血单个核细胞DNA损伤,可能与外周血CYP1A1基因表达水平和EROD活力下调有关。 Objective To explore the association between polycyclic aromatic hydrocarbons(PAHs)exposure and cytochrome P450 CYP1A1 expression at gene and enzyme activity levels in the peripheral blood monocyte cells in coke oven workers,and to provide a certain basis for the biological monitoring of health damage in coke oven workers.Methods We surveyed 118 coke oven workers and 63 controls(energy power workers in the same company)using self-designed questionnaire,determined their post-shift urinary 1-hydroxypyrene(1-OH-Py)concentration using high performance liquid chromatography(HPLC)-fluorescence detector method.We also isolated the peripheral blood mononuclear cell(PBMC)from fasting venous blood,and detected DNA damage using comet assay,CYP1A1 mRNA level using the real-time fluorescent quantitative PCR(FQ-PCR),and EROD activity using spectrophotometry.Statistical analyses including one-way analysis of variance and multiple linear regressions were used to analyze the association of urinary 1-OH-Py and CYP1A1 mRNA level and EROD activity.Results Compared to the control group,the urinary 1-OH-Py concentration and PBMC DNA tail moment were significantly increased in coke oven workers(P<0.05),and CYP1A1 gene level and EROD activity in PBMC were significantly decreased(P<0.05).Multiple linear regression showed that a ten-fold increase of urinary 1-OH-Pycon centration was associated with a decrease of 0.77(95%CI:-1.33--0.21)in CYP1A1 gene level,and a decline of 0.15(95%CI:-0.76--0.16)in EROD activity of PBMC in coke oven workers(P<0.05).Conclusion Occupational PAHs exposure induced DNA damage,which was associated with the decreased level in CYP1A1 gene cavel and EROD activity in PBMC of coke oven workers.
作者 李新 张红梅 钱波 高雁萍 吴苗 潘宝龙 王清花 杨可达 Li Xin;Zhang Hongmei;Qian Bo;Gao Yanping;Wu Miao;Pan Baolong;Wang Qinghua;Yang Keda(Taiyuan 030003,China;School of Public Health,Shanxi Medical University,Taiyuan 030001,China)
出处 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2019年第2期104-109,共6页 Chinese Journal of Industrial Hygiene and Occupational Diseases
基金 太原钢铁(集团)有限公司总医院科研项目(201411、201802) 山西省研究生联合培养基地人才培养项目(2017JD24) 山西省回国留学人员科研资助项目(2017-059).
关键词 外周血单个核细胞 DNA损伤 CYP1A1基因 酶活力 Peripheral blood monocyte cell DNA damage CYP1A1gene Enzyme activity
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