Objective: To investigate the effect of chlorhexidine gargle combined with orthodontic treatment on anterior teeth displacement caused by periodontal disease and the effects of chlorhexidine gargle on serum levels of interleukin-2, interleukin-6 and tumor necrosis factor-alpha. Methods: 108 patients with anterior teeth displacement caused by periodontal disease were selected from January 2015 to January 2018 in our hospital. They were divided into two groups by simple random number table. The control group (54 cases) was treated with orthodontic treatment, while the observation group (54 cases) was treated with chlorhexidine mouthwash. The clinical efficacy, periodontal indexes, serum levels of IL-2, IL-6 and TNF-alpha, and adverse reactions were compared between the two groups before and after treatment. Results: After receiving treatment for 12 months, overall effective rate of observation group was 94.4% which was significant higher than control group's 81.5%(P<0.05). After receiving treatment for 12 months, Two groups' periodontal index including probing bleeding rate, anterior teeth covering, periodontal pocket depth values and serum IL-2, IL-6 and TNF-α decreased significantly if comparing with those before treatment (P<0.01), and observation group's above index decreased more significantly (P<0.01). Serum IL-2 of two groups' increased significantly after receiving treatment for 12 months if comparing with that before treatment (P<0.01). Observation group's serum IL-2 was significant higher after treatment if comparing with control group at same time (P<0.01). There was no statistical significance if comparing control group adverse reaction rate (13.0%) with observation group's (9.3%)(P>0.05). Conclusion: The treatment of anterior teeth displacement caused by periodontal disease with chlorhexidine mouthwash combined with oral malformation can effectively regulate the level of inflammatory cytokines in patients, improve periodontal status and have significant clinical effects.