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女贞子多糖对仔猪抗氧化及MAPK通路的影响 预览

Studies on Antioxidant and MAPK Pathway of Ligustrum Polysaccharides in Piglets
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摘要 为了研究女贞子多糖对人工感染大肠杆菌K88仔猪氧化应激的保护作用,将实验猪分为四组:对照组、模型组、女贞子多糖组和女贞子多糖饲喂后攻毒组。试验运用血清生化、ELISA和RT-PCR等技术,通过检测血清和肝肾组织的抗氧化酶和氧化产物,仔猪肝脏氧化应激相关基因转录水平和MAPK信号关键蛋白的转录,评价女贞子多糖的抗氧化机制。结果显示,女贞子多糖可以显著降低由大肠杆菌引起的仔猪血清MDA含量的升高,调节肝肾组织中MDA和SOD的水平;女贞子多糖可以提高仔猪肝脏中CAT和CuZnSOD的mRNA转录水平;正常情况下,女贞子多糖对仔猪肝脏TNF-α的mRNA转录表达无影响,但可以降低大肠杆菌攻毒后的转录水平;女贞子多糖抑制MAPK通路主要依靠调控ERK1起作用。结果表明,女贞子多糖可以通过调节肝脏抗氧化指标保护大肠杆菌引起的仔猪氧化应激反应,其作用分子机制可能与下调MAPK相关信号转录水平有关。 In order to study the protective effect of ligustrum polysaccharide on oxidative stress of artificially infected piglets with E.coli K88, The experimental pigs were divided into four groups: control, model, ligustrum polysaccharide and ligustrum polysaccharide with E.coli .In this study, serum biochemical, ELISA and RT-PCR were used to detect antioxidant enzymes and oxidative products in serum and liver and kidney tissues, transcription level of genes related to liver oxidative stress in piglets, transcription of key proteins of MAPK signal, and antioxidant mechanism of ligustrum polysaccharide. The results showed that the ligustrum polysaccharide could significantly reduce the content of MDA in piglets caused by E.coli and regulate the level of MDA and SOD in liver and kidney tissues. The mRNA transcription of CAT and CuZnSOD in the livers of piglets can be improved by the polysaccharides of livers. Under normal circumstances, the mRNA expression of TNF-α in the liver of piglets was not affected by the polysaccharides of the livers, but the expression of the transcription of E.coli after challenge was reduced.Ligustrum polysaccharide inhibited activation of MAPK pathway mainly depends on regulation of ERK1. Conclusion: ligustrum polysaccharide can protect piglet oxidative stress response induced by E.coli by regulating liver antioxidant index, and its molecular mechanism may be related to down-regulation of MAPK related signal transcription level.
作者 朱琪 梁世岳 鲁森 赵骏 李玉鹏 乔家运 李海花 ZHU Qi;LIANG Shi-yue;LU Sen;ZHAO Jun;LI Yu-peng;QIAO Jia-yun;LI Hai-hua(Tianjin Institute of Animal Husbandry and Veterinarym,Tianjin 300381,China;Tianjin Livestock and Poultry Health Breeding Technology Engineering Center,Tianjin 300381,China;Tianjinuniversity,School of science,Tianjin 300072,China;Tianjin University of Traditional Chinese Medicine,School of Chinese Materia Medica,Tianjin 300381,China;Tianjin Agricultural University,College of animal science and animal medicine,Tianjin 300384,China)
出处 《中国兽药杂志》 2019年第4期65-71,共7页 Chinese Journal of Veterinary Drug
基金 十三五国家重点研发计划“畜禽养殖绿色安全饲料饲养新技术研发”(编号:2018YFD0500600) 天津市“131”创新型人才团队(编号:20180338).
关键词 女贞子多糖 仔猪 大肠杆菌病 抗氧化 MAPK通路 ligustrum polysaccharides piglets colibacillosis antioxidant MAPK pathway
作者简介 朱琪,博士,从事临床兽医学方向研究;通讯作者:李海花。E-mail:lihaihuaok@126.com.
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