Objective To investigate the feasibility of establishing hypertrophic scar animal model in mouse ear and abdomen by new modeling method. Methods 28 female ICR mice were divided into an experimental group (n=14) and a control group (n=14), and the control mice were left untreated.A corneal trepan was used to create a full-thickness defect (retaining cartilage) with Ф=3.5 mm in the ventral surface of both ears of the 14 mice in the experimental group, and then the mouse ears was sutured with absorbable surgical sutures. After the wound surface was epithelialized,the Gross,HE staining and Masson staining were performed and the proliferation was assessed by using a Vancouver quantitative table. Results 14 days after model-establishing,27 ears (27/28) of the mice in the experimental group developed hyperplasia.The degree of hyperplasia in 28 ears of these mice was evaluated according to Vancouver quantitative table,and the result showed that the score of thickness,vascular distribution,color and softness of ear scar in the experimental group was higher than that in the control group ( P <0.05).HE staining showed that hyperplasia of the auricular dermis was active in the experimental group,accompanied by a large number of connective tissue hyperplasia under the epidermis.The fibroblast layer of hypertrophic scar tissue in the experimental group was (61.8±42.5),which was higher than that in the control group (9.5±1.9)( P <0.05). Masson staining showed that in the experimental group,a large amount of collagen deposition was generated, which was organized disorderly,between hyperplasia cells of the dermis in the scar area,and the collagenous fibers were thick and dense. Conclusion The mouse hypertrophic scar model established in this study has a high success rate,simple and economical method and short modeling time.
JOurnal of Xiangnan University:Medical Sciences