为开发适宜于紫苏的SSR标记,对紫苏遗传多样性及种群划分进行研究,本研究采用已报道的紫苏及近源种中的66对SSR引物对不同来源的42份紫苏材料进行扩增。结果表明:15对引物在42份材料中具有多态性,共扩增获得54个条带,平均每对引物扩增条带3.6个,51个条带具有多态性,占扩增总条带的94.4%。使用UPGMA方法进行聚类分析,在SM=0.67处,供试紫苏分为2个主要聚类群。类群Ⅰ为紫苏原变种(Perilla frutescens var. frutescents),类群Ⅱ为回回苏变种(Perilla frutescens var. crispa),类群Ⅰ又可进一步划分为5个亚类群,各亚类群间表型具有一定的特征差异。本研究可为紫苏种质亲缘关系及资源鉴定研究提供参考依据。
In order to develop SSR markers suitable for Perilla frutescens and study the genetic diversity and population division of P. frutescens, in this study, 66 pairs of SSR primers from Perilla and its relatives were used to amplify 42 Perilla materials from different sources. The results showed that: 15 pairs of primers were polymorphic in 42 materials, 54 bands were obtained by amplification, with an average of 3.6 bands per pair of primers, and 51 bands were polymorphic, accounting for 94.4% of the total amplification bands. The UPGMA method was used to cluster analysis. At SM=0.67, the P. frutescens was divided into two main clustering groups.GroupⅠ was P. frutescens var. frutescents, GroupⅡ was P. frutescens var. crispa and GroupⅠ could be further divided into five subgroups. The phenotypes of each subgroup had a certain characteristic difference. The results of this study could provide reference for the research of the Perilla genetic relationship and resources identification.
Molecular Plant Breeding