Objective: To investigate the expression of neutrophil gelatinase-associated lipid carrier protein (NGAL) in serum,umbilical cord blood and placenta of gestational diabetes mellitus (GDM) and its correlation with neonatal weight. Methods: From Jun. 2017 to Nov. 2017,49 patients with GDM underwent cesarean section due to scar uterus and breech position in Hebei General Hospital and 39 age-matched normal controls were recruited.The levels of serum NGAL in maternal and umbilical blood were measured by ELISA.The expressions of NGAL mRNA and its protein in placenta tissue were detected by RT-PCR and Western blot. Results: The levels of serum NGAL in maternal and umbilical blood in GDM group were higher than those in NGT group,and the expression of NGAL mRNA and protein in placental tissue of GDM patients were higher than those in NGT group.The difference between the two groups was statistically significant ( P <0.01).There was a positive correlation between maternal NGAL level and umbilical cord blood in NGT group and GDM group[( r =0.399, P =0.012)、( r = 0.349 , P =0.014)].The maternal NGAL level in GDM group was positively correlated with the levels of NGAL protein in placental tissue ( r =0.636, P =0.011),mRNA ( r =0.848, P = 0.008 ),neonatal weight( r =0.363, P =0.014)and insulin resistance index ( r =0.312, P = 0.044 ). Conclusions: The increase of NGAL level in peripheral blood of GDM patients may be mainly due to the over-secretion of NGAL factor in placenta,and may participate in the development of GDM by increasing insulin resistance.NGAL can be transported through the placenta to the fetus and further affects the fetal intrauterine growth and development.
Current Advances In Obstetrics and Gynecology
Gestational diabetes mellitus
Neutrophil gelatinase-associated lipid carrier protein
Fetal intrauterine growth and development