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封丘金银花炭疽病病原的分离和鉴定 预览

Isolation and identification of honeysuckle anthracnose pathogen in Fengqiu
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摘要 为明确河南封丘金银花炭疽病病原种类,对采自封丘的金银花炭疽病病叶,依据柯赫氏法则进行分离、纯化、回接和再分离,证明获得的分离菌株是金银花炭疽病的致病菌.对其培养性状、在寄主上的形态特征及萌发方式进行观察,并对其rDNA-ITS序列进行分析,结果表明:致病菌株在PDA培养基上菌落呈白色,后期菌落中央颜色变暗,生分生孢子盘;分生孢子盘具黑色刚毛,分生孢子长圆柱形,两端钝圆,单细胞.核糖体DNA-ITS序列与GenBank数据库胶孢炭疽菌同源性达99%.将封丘金银花炭疽病病原菌鉴定为胶孢炭疽菌Colletotrichum gloeosporioides. In order to determine the pathogen of Lonicera japonica anthracnose in Henan Province,infected leaves were collected from Fengqiu,Henan Province.The pathogen was isolated,back-inoculated,re-isolated by Koch’s postulates and was identified according to morphological and culture characteristics and the sequence of ribosomal DNA-ITS.The results showed that the fungus colonies on PDA were white,later pale yellow.Acervuli with setae formed in the center of the colonies.Conidia long cylindrical,obtuse at both ends,single-celled.rDNA-ITS sequence analysis indicated that the pathogen and Colletotrichum gloeosporioidesisolate COL4 were 99%in homology.he pathogen was identified as Colletotrichum gloeosporioides.
作者 李志红 翟凤艳 张永霞 王迪 邢登可 鲁海菊 刘英杰 LI Zhihong;ZHAI Fengyan;ZHANG Yongxia;WANG Di;XING Dengke;LU Haiju;LIU Yingjie(School of Resource and Environmental Sciences,Henan Institute of Science and Technology,Xinxiang 453003,China;Department of Life Science and Technology,Honghe College,Mengzi 661199,China)
出处 《河南科技学院学报:自然科学版》 2019年第2期20-23,共4页 Journal of Henan Institute of Science and Technology
基金 国家大学生创新训练项目(2018CX29) 河南省高等学校青年骨干教师培养计划项目(2016GGJS109).
关键词 金银花 炭疽病 胶孢炭疽菌 rDNA-ITS序列 鉴定 honeysuckle anthracnose Colletotrichum gloeosporioides rDNA-ITS sequence idendification
作者简介 李志红(1997-),男,河南林州人,本科生;通信作者:翟凤艳(1978-),女,吉林吉林人,博士,副教授,硕士生导师.主要从事病原真菌系统分类学研究.
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