期刊文献+

柴贝止痫汤及其主要入血成分柴胡皂苷a对癫痫模型大鼠卡马西平脑内分布的影响

Effects of Chaibei Zhixian Decoction (柴贝止痫汤)and its Constituents Migrating to Blood Saikosaponin a on Distribution of Carbamazepine in Brain in Epilepsy Model Rats
分享 导出
摘要 目的观察柴贝止痫汤及柴胡皂苷a治疗难治性癫痫的可能作用机制。方法 88只大鼠随机分为空白组12只及模型组、卡马西平组、柴贝止痫汤+卡马西平组、柴胡皂苷a+卡马西平组19只。除空白组外,其余各组采用侧脑室注射海人酸制备癫痫大鼠模型。卡马西平组给予卡马西平混悬液0.75 ml/(100 g·d),柴贝止痫汤+卡马西平组给予柴贝止痫汤0.5 ml/(100 g·d)和卡马西平混悬液0.75 ml/(100 g·d),柴胡皂苷a+卡马西平组给予柴胡皂苷a混悬液0.25 ml/100 g和卡马西平混悬液0.75 ml/100 g,模型组及空白组给予等量羧甲基纤维素钠混悬液。给药60天后比较各组海马P糖蛋白、Mdr1a mRNA表达及全脑卡马西平(CBZ)、1011-环氧化卡马西平(CBZE)含量。结果与空白组比较,模型组海马P糖蛋白表达升高(P<0.05);与模型组比较,卡马西平组、柴胡皂苷a+卡马西平组海马P糖蛋白表达差异无统计学意义(P>0.05),柴贝止痫汤+卡马西平组海马P糖蛋白表达降低(P<0.05);与卡马西平组比较,柴贝止痫汤+卡马西平组和柴胡皂苷a+卡马西平组海马P糖蛋白表达均降低(P<0.05),柴贝止痫汤+卡马西平组和柴胡皂苷a+卡马西平组海马P糖蛋白表达差异无统计学意义(P>0.05)。模型组Mdr1a mRNA表达较空白组升高。卡马西平组的Mdr1a mRNA表达高于空白组(P<0.05),空白组、模型组MDR1a mRNA表达分别为卡马西平组的0.36倍、0.87倍。柴贝止痫汤+卡马西平组较卡马西平组Mdr1a含量降低(P<0.01),为卡马西平组的0.14倍。与卡马西平组比较,柴贝止痫汤+卡马西平组CBZE含量升高(P<0.01),柴胡皂苷a+卡马西平组CBZE含量降低(P<0.01),且柴贝止痫汤+卡马西平组和柴胡皂苷a+卡马西平组CBZ差异无统计学意义(P>0.05);与柴贝止痫汤+卡马西平组比较,柴胡皂苷a+卡马西平组CBZ、CBZE含量均降低(P<0.01)。结论柴贝止痫汤联合卡马西平可降低癫痫模型大鼠海马Mdr1a/P糖蛋白表达,促进 Objective To observe the possible mechanism of Chaibei Zhixian Decoction( CBZXD,柴贝止痫汤)and Saikosaponin a( SSa) in treating intractable epilepsy. Methods A total of 88 rats were randomly divided into blank group of 12 rats and every 19 rats for model group,carbamazepine( CBZE) group,the CBZXD + CBZE group,and the saikosaponin a + CBZE group. Except for the blank group,other groups used the lateral ventricle injection of kainate to prepare a rat model of epilepsy. The CBZE group was given CBZE suspension 0. 75 ml/( 100 g·d). The CBZXD + CBZE group was given CBZXD 0. 5 ml/( 100 g·d) and CBZE suspension 0. 75 ml/( 100 g·d).The saikosaponin a + CBZE group was given saikosaponin a suspension 0. 25 ml/100 g and CBZE suspension0. 75 ml/100 g. The model group and the blank group were given the same amount sodium carboxymethylcellulose suspension. After 60 days of administration,the expression of P-glycoprotein,Mdr1 a mRNA and the content of whole brain carbamazepine( CBZ) and 10 11-epoxidized CBZE were compared. Results Compared with the blank group,the expression of P-glycoprotein in the hippocampus of the model group was increased( P < 0. 05). Compared with the model group,the expression of P-glycoprotein in the hippocampus of the CBZE group,saikosaponin a + carbazepine group was not statistically significant( P > 0. 05). The expression of P-glycoprotein in hippocampus of CBZXD + CBZE group was decreased( P < 0. 05). Compared with CBZE group,the expression of P-glycoprotein in hippocampus of CBZXD + CBZE group and saikosaponin a + CBZE group was decreased( P < 0. 05). There was no significant difference in the expression of P-glycoprotein in hippocampus between CBZXD + CBZE group and saikosaponin a + CBZE group( P > 0. 05). Mdr1 a mRNA expression in the model group was increased compared with that in the blank group. The expression of Mdr1 a mRNA in CBZE group was higher than that in the blank group( P <0. 05). The expression of MDR1 a mRNA in blank group and model group was 0. 36 times and 0. 87 times h
作者 孙江燕 刘冲冲 袁斯远 王潇慧 刘金民 SUN Jiangyan;LIU Chongchong;YUAN Siyuan;WANG Xiaohui;LIU Jinmin(Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029;Dongfang Hospital, Beijing University ofChinese Medicine;Qingdao Hiser Hospital)
出处 《中医杂志》 CSCD 北大核心 2019年第8期689-695,共7页 Journal of Traditional Chinese Medicine
基金 国家自然科学基金(81774277) 北京中医药大学研究生校级自主课题(2018-JYBZZ-XS239).
关键词 难治性癫痫 柴贝止痫汤 柴胡皂苷A P糖蛋白 Mdr1a 卡马西平 10 11-环氧化卡马西平 intractable epilepsy Chaibei Zhixian Decoction(柴贝止痫汤) saikosaponin a P-glycoprotein Mdr1a carbamazepine 10,11-epoxidation of carbamazepine
作者简介 通讯作者:刘金民,jmvip@vip.163.com,(010)67689618.
  • 相关文献

参考文献9

二级参考文献50

共引文献59

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈