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云南省西双版纳州狂犬病流行及狂犬病病毒遗传进化特征

Epidemiological features of rabies and molecular evolution characteristics of the rabies virus strains in Xishuangbanna prefecture of Yunnan province, China
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摘要 目的阐明云南省西双版纳傣族自治州(西双版纳州)2008—2017年狂犬病流行病学特点和狂犬病病毒流行株遗传进化特征。方法收集狂犬病病例资料,采集狂犬和可疑犬脑组织以及狂犬病患者唾液、脑脊液和尸检脑组织标本,用反转录聚合酶链式反应扩增狂犬病病毒核蛋白和糖蛋白基因核苷酸序列,用相关生物学软件进行同源性和进化分析。结果2008—2017年西双版纳州所辖3个县的28个乡镇(办事处、农场)共发生狂犬病62例,其中景洪市、勐海县和勐腊县分别为37例、15例和10例。其中48例被家犬咬伤(77.42%),11例被流浪犬咬伤(17.74%)。这10年间每年都有病例发生,季节性发病不明显;年龄以30~59岁为主,最小1岁,最大91岁;男女性别比为1.70∶1,以农民为主。获得9株狂犬病病毒的核蛋白基因核苷酸序列(景洪7株、勐海1株和勐腊1株),进化和同源性分析表明,5株为China-Ⅰ进化群、3株为China-Ⅱ和1株为China-Ⅵ。还获得5株狂犬病病毒的糖蛋白基因序列(景洪3株、勐海1株和勐腊1株),均为China-Ⅰ进化群,与这5株的核蛋白基因分析结果一致。西双版纳China-Ⅰ和China-Ⅱ狂犬病病毒均与近几年相邻普洱市以及四川、贵州和广西相应进化群流行株具有较近的遗传进化关系;China-Ⅵ与滇西南的China-Ⅵ以及邻国缅甸、老挝和越南流行株具有较高同源性和亲缘性。结论西双版纳狂犬病主要流行于农村地区,家养犬为主要传染源。当地存在狂犬病病毒的China-Ⅰ、China-Ⅱ和China-Ⅵ进化群,以China-Ⅰ为主;China-Ⅰ和China-Ⅱ的传播来源为相邻地区,China-Ⅵ的传播来源为缅甸和老挝。 Objective To understand the epidemiologic features of the rabies in Xishuang banna prefecture of Yunnan province, China in 2008-2017 and the viral molecular-evolution characteristics. Methods The data of rabies case questionnaire were collected. The brain tissue samples from mad dogs, suspicious sick dogs and human brain tissue, saliva and cerebrospinal fluid samples from rabies patients were collected in Xishuangbanna. Coding region of nucleoprotein and glycoprotein genes were amplified by RT-PCR and sequenced. Homology and phylogenetic analysis were performed using the relevant bioinformatics software. Results A total of 62 cases of human rabies were occurred in 28 districts of the 3 counties, Xishuangbanna prefecture in 2008-2017. Of them, 37 cases in Jinghong county, 15 in Menghai county and 10 in Mengla county. In which 48 cases were bitten by domestic dogs (77.42%), 11 cases were bitten by wild dogs (17.74%). Rabies case was occurred every year in the past decade. The seasonal incidence was not obvious. The majority of patients were aged from 30 to 59 years-old, with the youngest 1 year-old and the eldest 91 year-old. The male to female ratio was 1.70∶1, most cases were farmers. The nucleotide sequences of nucleoprotein gene of 9 virus strains (7 from Jinghong, 1 from Menghai and 1 from Mengla) were obtained from the samples of dogs and patients. Homology and phylogenetic analyses indicated that the 5 strains belonged to clade China-Ⅰ, 3 clade China-Ⅱ and 1 clade China-Ⅵ. The nucleotide sequences of glycoprotein gene of 5 virus strains (3 from Jinghong, 1 from Menghai and 1 from Mengla) were obtained from these positive samples, and all were clade China-Ⅰ, it is same with nucleoprotein genes analysis result from these 5 virus strains. These China-Ⅰ and China-Ⅱ strains from Xishuangbanna have a closer genetic relationship with same clade strains isolated from Pu’er and other prefectures of Yunnan province as well as Sichuan, Guizhou and Guangxi. The China-Ⅵ strain from Xishuangbanna share h
作者 杨红梅 冯云 来明月 李保华 范建华 马龙 文红华 许云桥 刀应华 苏梅惠 刘华兴 章域震 杨卫红 张海林 Yang Hongmei;Feng Yun;Lai Mingyue;Li Baohua;Fan Jianhua;Ma Long;Wen Honghua;Xu Yunqiao;Dao Yinghua;Su Meihui;Liu Huaxing;Zhang Yuzhen;Yang Weihong;Zhang Hailin(Xishuangbanna Prefecture Center for Disease Control and Prevention, Jinghong 666100, China;Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali 671000, China;Jinghong City Center for Disease Control and Prevention, Jinghong 666100, China;Menghai County Center for Disease Control and Prevention, Menghai 666200, China;Mengla County Center for Disease Control and Prevention, Mengla 666300, China)
出处 《中华实验和临床病毒学杂志》 CAS CSCD 2019年第2期113-120,共8页 Chinese Journal of Experimental and Clinical Virology
关键词 狂犬病 狂犬病病毒 进化分析 同源性分析 流行病学 Virus disease Rabies Rabies virus Phylogenetic analysis Homology analysis Epidemiology
作者简介 杨红梅和冯云对本文有同等贡献;杨红梅和冯云对本文有同等贡献;通信作者:张海林,Email:zhangHL715@163.com,电话:0872-2196147.
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