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两种商品化培养液对胚胎体外发育和临床结局的影响比较 预览

Comparison of in vitro embryo development and clinical outcomes with two commercial culture media applied in IVF-ET and ICSI-ET
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摘要 目的 比较Cook培养液和Vitrolife培养液对胚胎体外发育和临床结局的影响。方法 收集行常规体外受精-胚胎移植(IVF-ET)893例和卵胞浆内单精子注射-胚胎移植(ICSI-ET)320例新鲜周期的临床数据。根据使用的培养液不同,IVF-ET周期分为Cook组456例和Vitrolife组437例,ICSI-ET周期分为Cook组143例和Vitrolife组177例。分别比较IVF-ET周期和ICSI-ET周期中Cook组和Vitrolife组获卵个数、正常受精率、卵裂率、第3天优质胚胎率、ET个数、种植率、妊娠率。结果 IVF-ET和ICSI-ET周期中,Cook组和Vitrolife组获卵个数、正常受精率、卵裂率、ET个数、种植率、妊娠率比较差异均无统计学意义(均P >0.05),但Vitrolife组第3天优质胚胎率均明显高于Cook组,差异均有统计学意义(均P<0.01)。结论 无论IVF-ET还是ICSI-ET周期,两种培养液对种植率和妊娠率均没有影响,但Vitrolife培养液可以获得更高的第3天优质胚胎率。 Objective To compare the effects of Cook and Vitrolife culture media on in vitro embryo development and clinical outcomes of IVF-ET and ICSI-ET. Methods A total of 893 cycles of IVF-ET and 320 cycles of ICSI-ET in our reproductive center were analyzed retrospectively.In IVF-ET, 456 cycles used Cook media and 437 cycles used Vitrolife media, while for ICSI-ET there were 143 cycles with Cook media and 177 cycles with Vitrolife media, respectively. The number of retrieved oocytes, fertilization rate, cleavage rate, the top quality embryos, number of ET, implantation rate and pregnancy rates were compared between Cook group and Vitrolife group in IVF-ET or ICSI-ET cycles. Results There was no significant difference in the number of retrieved oocytes, fertilization rate, cleavage rate, number of ET, implantation rate and pregnancy rate between the two groups(all P >0.05), while the top quality embryos rate of Vitrolife group was higher than that of Cook group, both in IVF-ET and ICSI-ET cycles(both P<0.01). Conclusion There are no significant differences in implantation rate and pregnancy rate between Cook and Vitrolife culture media used in IVF-ET and ICSI-ET cycles. However, the Vitrolife media may be better for the improvement in embryo quality than Cook media.
作者 潘承双 徐芝慧 张欢 李燕 金建远 费前进 倪吴花 PAN Chengshuang;XU Zhihui;ZHANG Huan(Reproductive Medicine Center, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China)
出处 《浙江医学》 CAS 2019年第10期1041-1044,共4页 Zhejiang Medical Journal
基金 浙江省自然科学基金项目(LQ16H040003) 浙江省人口计划生育科研项目(2014KYB340) 温州市科技计划项目(Y20140728).
关键词 体外受精 胚胎培养 培养液 In vitro fertilization Embryo culture Culture medium
作者简介 通信作者:倪吴花,E-mail:niwuhua228@163.com.
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