期刊文献+

基于RNA-Seq技术筛选影响猪肌纤维性状的候选基因 预览

Screening of Candidate Genes for Muscle Fiber Characteristics in Pig Using RNA-Seq
在线阅读 免费下载
收藏 分享 导出
摘要 旨在探究影响肌纤维性状的候选基因和信号通路。本研究以马身猪和大白猪为研究对象,采用HE染色法分析6月龄马身猪和大白猪背最长肌肌纤维直径和密度,利用RNA-Seq分析马身猪和大白猪背最长肌组织中基因表达情况,并对差异表达基因进行GO和KEGG富集分析,再通过qRT-PCR验证RNA-Seq结果的准确性。结果显示,马身猪背最长肌肌纤维直径极显著低于大白猪(P<0.01),而肌纤维密度极显著高于大白猪(P<0.01)。转录组测序结果显示,在6月龄马身猪和大白猪背最长肌中,差异倍数在2倍以上的基因共105个,其中,马身猪相对于大白猪上调的基因有55个,下调的基因有50个。GO富集分析表明,差异表达基因主要富集在与线粒体相关的细胞组分和骨骼肌分化有关的生物学过程中;KEGG分析结果显示,这些差异表达基因主要参与到与氧化磷酸化有关的信号途径。qRT-PCR和RNA-Seq对6个DEGs表达情况的检测结果表明它们的表达趋势相同,说明RNA-Seq结果准确可靠。结合差异基因表达丰度、GO和KEGG富集分析结果,本研究发现,MYL3、MYH3、MYH6基因通过影响肌纤维的组成而影响肌纤维特性,ND6基因通过参与线粒体氧化磷酸化过程影响肌纤维类型,MICU2基因通过维持线粒体Ca2+浓度的稳态影响细胞功能,编码转录因子的EGR1和FOS基因通过调节细胞增殖、分化和凋亡等过程影响肌肉生长发育。本研究初步揭示了造成马身猪和大白猪肌纤维性状差异的主要原因,为猪肉品质的改善提供了相应的理论依据。 The aim of this study was to explore the candidate genes and signal pathways affecting muscle fiber characteristics.In this study,muscle fiber diameter and density of longissimus dorsi of Mashen and Large White pigs aged 6 months were measured by HE staining,and the gene expressions in longissimus dorsi of the 2 pig breeds at 6 months old was detected by using RNA-Seq.GO and KEGG enrichment analysis were used to study the function of differentially expressed genes(DEGs).The accuracy of RNA-Seq results was verified by qRT-PCR.The results showed that the fiber diameter of longissimus dorsi of Mashen pig was significantly shorter than that of Large White pig ( P <0.01),and the fiber density of Mashen pig was greater than that of Large White pig ( P <0.01).A total of 105 DEGs at least 2-fold change were identified,including 55 up-regulated genes and 50 down-regulated genes in longissimus dorsi of Mashen pig compared to Large White pig.GO enrichment analysis showed that the DEGs were mainly enriched in the cellular component related to mitochondrion and skeletal muscle differentiation biological processes,and KEGG analysis showed that these DEGs were mainly involved in the signaling pathways related to oxidative phosphorylation.The expression trends of 6 selected DEGs detected by qRT-PCR and RNA-Seq were the same,which indicated that the results of RNA-Seq were accurate and reliable.Based on the results of expression abundance,GO and KEGG analysis of DEGs,the genes of MYL 3,MYH 3 and MYH 6 were found to affect muscle fiber characteristic by influencing the composition of muscle fiber.ND 6 gene was related to muscle fiber type by involving in oxidative phosphorylation pathway in mitochondria.MICU 2 gene was involved in keeping homeostasis of Ca^2+ concentration in mitochondria and further affected cellular function.The genes of EGR 1 and FOS coding transcription factors might affect muscle growth and development by regulating cell proliferation,differentiation and apoptosis.This study preliminarily explored the reason
作者 成志敏 张宁芳 王媛媛 乐宝玉 张万锋 宋鹏康 郭晓红 高鹏飞 蔡春波 曹果清 李步高 CHENG Zhimin;ZHANG Ningfang;WANG Yuanyuan;LE Baoyu;ZHANG Wanfeng;SONG Pengkang;GUO Xiaohong;GAO Pengfei;CAI Chunbo;CAO Guoqing;LI Bugao(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2019年第5期918-929,共12页 Chinese Journal of Animal and Veterinary Sciences
基金 国家自然科学基金(31872336) 山西省科技创新重点团队(201605D131045-24) 三晋学者支持计划专项经费(2016 2017) “山西省1331工程”.
关键词 肌纤维性状 候选基因 RNA-SEQ pig muscle fiber characteristics candidate genes RNA-Seq
作者简介 成志敏(1993-),男,山西襄汾人,硕士,主要从事动物遗传育种与繁殖研究,E-mail:czmznf12@163.com;通信作者:李步高,主要从事动物遗传资源分子评定与利用研究,E-mail:jinrenn@163.com.
  • 相关文献

参考文献5

二级参考文献87

  • 1郑洁,邝良德,张翔宇,杨超.兔肌纤维组织特性对肉品质影响的研究进展[J].中国养兔,2014,0(4):31-33. 被引量:1
  • 2符金录 ,刘汉卿 .秦川牛肉用性能开发前景及对策[J].黄牛杂志,2005,31(5):39-41. 被引量:1
  • 3魏法山,康相涛,李国喜,孙桂荣,韩瑞丽,宋素芳,李明,王彦彬,黄艳群,乔欣君,闫峰宾.固始鸡生长过程中不同类型肌纤维面积比的变化[J].西北农林科技大学学报:自然科学版,2006,34(2):7-11. 被引量:12
  • 4GB 5797-2003,秦川牛.北京:中国标准出版社,2003. 被引量:1
  • 5Li C,Wong WH.Model-based analysis of oligonucleotide arrays:Expression index computation and outlier detection.Proc Natl Acad Sci USA,2001,98(1):31-36. 被引量:1
  • 6Li C,Wong WH.Model-based analysis of olignnucleotide arrays:model validation,design issues and standard error application.Genome Biol,2001,2(8):research0032.1-0032.11. 被引量:1
  • 7Tusher VG,Tibshirani R,Chu G.Significance analysis of microarrays applied to the ionizing radiation response.Proc Natl Acad Sci USA,2001,98(9):5116-5121. 被引量:1
  • 8Qin LX,Beyer RP,Hudson FN,Linford NJ,Morris DE,Kerr KF.Evaluation of methods for oligonucleotide array data via quantitative real-time PCR.BMC Bioinformatics,2006,7:23-34. 被引量:1
  • 9Field,RA.Effect of castration on meat quality and quantity.J Anim Sci,1971,32(5):849-858. 被引量:1
  • 10Ende C,Gebhardt R.Inhibition of matrix metalloproteinase-2 and-9 activities by selected flavonoids.Planta Med,2004,70(10):1006-1008. 被引量:1

共引文献17

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈