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酶联免疫法检测花生样本中的黄曲霉毒素B1 预览

Detection of Aflatoxin B 1 in Peanut Samples by Enzyme Linked Immunosorbent Assay
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摘要 使用浓度为40%的甲醇-水对花生样本中的黄曲霉毒素B1(Aflatoxin B1,AFB1)进行提取,再做1:5稀释,采用酶联免疫吸附法(Enzyme-linked Immunosorbent assay,ELISA)对样本中AFB1含量进行检测,并验证该方法的线性,重现性、回收率。结果表明,AFB1在2.5~75μg/kg范围内线性关系较好,线性回归方程为:y=-0.507x+0.413,相关系数R2=0.999。重现性实验所得的相对标准偏差(RSD%)为1.56%,不同加标实验的加标回收率均大于97%,相对标准偏差(RSD%)分别为2.07%、3.73%、4.85%。本方法重现性好,准确度高,适用于高效的定量检测花生样本中的AFB1含量。 AFB 1 in peanut samples was extracted with 60% methanol-water and processed with 1 : 5 dilution, and then the content of AFB 1 in samples was determined by ELISA. The linearity, sensitivity, recovery and reproducibility of the peanut samples were verified. The results showed that the linear range of aflatoxin B 1 standard was 2.5~75 μg/kg, y=-0.507x+0.413, R 2=0.999. The relative standard deviation obtained from the reproducibility experiment was 1.56%. Recovery rate was over 97%, the relative standard deviation was 2.07%, 3.73% and 4.85%. This method is reproducible and accurate, which is suitable for efficient quantitative detection detection of aflatoxin B 1 in peanut samples.
作者 黄钟标 郑思珩 吴思敏 金佳佳 严家俊 HUANG Zhong-biao;ZHENG Si-hang;WU Si-min;JIN Jia-jia;YAN Jia-jun(Guangdong Testing Institute of Product Quality Supervision, Guangdong Foshan 528300;State Key Laboratory of Alcohol Testing(Guangdong) , Guangdong Guangzhou 510730, China)
出处 《广州化工》 CAS 2019年第9期124-125,136共3页 GuangZhou Chemical Industry and Technology
关键词 酶联免疫法 黄曲霉毒素B1 花生样品 enzyme-linked immunosorbent assay(ELISA) aflatoxin B 1 peanut samples
作者简介 第一作者:黄钟标(1992-),男,助理工程师,研究方向为食品质量与安全;通讯作者:严家俊(1992-),男,工程师,研究方向为食品质量与安全。
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