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大豆染料木素抑制小鼠结肠肿瘤发生过程中PPAR-γ的作用和机制

ROLE OF PPAR-γ DURING THE PROCESS OF COLON CANCER INHIBITION BY GENISTEIN IN MICE
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摘要 目的研究染料木素(Genistein)抑制小鼠结肠肿瘤发生过程中,过氧化物酶增殖物激活受体(PPAR-γ的作用和机制。方法用DSS/AOM法诱导雌性KM小鼠结肠肿瘤发生,小鼠共30只,分成正常对照组(normal,N)、结肠肿瘤诱导组(DSS/AOM)和日粮+Genistein(450mg/kg)的结肠肿瘤诱导组(DSS/AOM+Gen)三组,每组10只。分析小鼠结肠肿瘤的发生情况和健康状况,用Western-blot测定小鼠结肠组织相关蛋白的表达变化。结果 Genistein的添加明显降低了小鼠结肠肿瘤发生率,改善了结肠组织结构。结肠肿瘤小鼠PPARγ及PAT家族蛋白,包括Perilipin、TIP-47和ADRP等蛋白的表达显著提高,肿瘤抑制因子FOXO3a蛋白的表达显著降低(P<0.05),Genistein的添加对上述蛋白的表达起到了显著的逆转作用(P<0.05)。结论 PPARγ蛋白在结肠肿瘤中过表达,而Genistein的添加,抑制了其表达,从而进一步抑制了PAT家族蛋白的表达,使LDs积累减少,FOXO3a蛋白的表达活性升高,最终抑制结肠肿瘤的发生发展。 Objective To investigate the role of peroxisome proliferators-activated receptor gamma (PPAR-γ) in colon cancer inhibition by genistein in mice. Methods Colon cancer was induced by DSS/AOM method in female KM mice. Thirty mice were divided into normal control group (Normal, N), colon cancer induction group (DSS/AOM) and colon cancer induction group with 450mg/kg of genistein in diets (DSS/AOM+Genistein450mg/kg)(n=10). The tumorigenicity and physical conditions of mice were analyzed. The protein expressions were determined by Western-blot. Results Genistein decreased the tumorigenicity and improved the structure of colon in mice significantly. The expressions of PPAR-γ and PAT family proteins including perilipin, TIP-47 and ADRP were significantly increased in mice with colon cancer (P<0.05), while the expression of tumor suppressor FOXO3a was significantly decreased (P<0.05). The addition of genistein could reverse the changes of these proteins expression significantly (P<0.05). Conclusion PPAR-γ and PAT proteins are over-expressed in colon cancer, which can be reversed by genistein and followed by decreased LDs accumulation and increased FOXO3a. This results in the inhibition of colon cancer.
作者 綦文涛 姚金丽 王春玲 QI Wen-tao;YAO Jin-li;WANG Chun-ling(Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,100037;College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,13 Street,Tianjin Economic-Technological Development Area (TEDA),Tianjin,300457,China)
出处 《营养学报》 CAS CSCD 北大核心 2019年第2期178-182,共5页 Acta Nutrimenta Sinica
基金 国家自然科学基金面上基金项目(No.31471591).
关键词 大豆染料木素 小鼠 结肠癌 过氧化物酶增殖物激活受体 脂滴 genistein mice colon cancer PPAR-γ lipid droplets
作者简介 通信作者:綦文涛(1977—),男,副研究员,博士;E-mail:qwt@chinagrain.org.
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  • 1Ioannis A Voutsadakis.Peroxisome proliferator 激活受体 -- 并且在 colorectal 癌症的 ubiquitin-proteasome 系统[J].世界胃肠肿瘤学杂志:英文版(电子版),2010(5):235-241. 被引量:1
  • 2Jullumstr E,Wibe A,Lydersen S. Colon cancer incidence,presentation,treatment and outcomes over 25 years[J].Colorectal Disease,2011,(05):512-518. 被引量:1
  • 3Manfredi S,Bouvier AM,Lepage C. Incidence and patterns of recurrence after resection for cure of colonic cancer in a well defined population[J].British Journal of Surgery,2006,(09):1115-1122. 被引量:1
  • 4Harris GJ,Church JM,Senagore AJ. Factors affecting local recurrence of colonic adenocarcinoma[J].Diseases of the Colon & Rectum,2002,(08):1029-1034. 被引量:1
  • 5Sjvall A,Granath F,Cedermark B. Loco-regional recurrence from colon cancer:a population-based study[J].Annals of Surgical Oncology,2007,(02):432-440. 被引量:1
  • 6Read TE,Mutch MG,Chang BW. Locoregional recurrence and survival after curative resection of adenocarcinoma of the colon[J].Journal of the American College of Surgeons,2002,(01):33-40. 被引量:1
  • 7Yun HR,Lee LJ,Park JH. Local recurrence after curative resection in patients with colon and rectal cancers[J].International Journal of Colorectal Disease,2008,(11):1081-1087. 被引量:1
  • 8Obrand DI,Gordon PH. Incidence and patterns of recurrence following curative resection for colorectal carcinoma[J].Diseases of the Colon & Rectum,1997,(01):15-24. 被引量:1
  • 9Kraemer M,Wiratkapun S,Seow-Choen F. Stratifying risk factors for follow-up:a comparison of recurrent and nonrecurrent colorectal cancer[J].Diseases of the Colon & Rectum,2001,(06):815-821. 被引量:1
  • 10Bowne WB,Lee B,Wong WD. Operative salvage for locoregional recurrent colon cancer after curative resection:an analysis of 100 cases[J].Diseases of the Colon & Rectum,2005,(05):897-909. 被引量:1

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