期刊文献+

不同中药药对调节氢化可的松药源性证候小鼠肾上腺皮质功能的研究

Effects of different Chinese meteria medica pair on regulation of adrenal function in mice with hydrocortisone-induced syndrome
分享 导出
摘要 目的研究不同治法中药配伍调节肾上腺皮质功能的作用。方法小鼠ig给予1.65 mg/(kg·d)氢化可的松14 d诱发药源性证候,同步给予助阳散寒(附子-肉桂)、滋阴泻火(知母-黄柏)、补气生津(人参-黄芪)、补血益精(熟地-山茱萸)4种中药药对干预,检测小鼠体质量、抓力、腋温、旷场活动度、躯体不同部位红外温度等表征信息;取脾脏、胸腺称定质量并计算脏器指数;运用ELISA法检测血清皮质酮、促肾上腺皮质激素(ACTH)、肾上腺素和去甲肾上腺素含量;采用实时荧光定量PCR技术检测肾上腺类固醇合成急性调节蛋白(StAR)、胆固醇侧链裂解酶(Cyp11a1)、21-羟化酶(Cyp21a1)、11β-羟化酶1(Cyp11b1)和11β-羟化酶2(Cyp11b2)、酪氨酸羟化酶(Th)、多巴胺脱羧酶(Ddc)、多巴胺β-羟化酶(Dbh)、苯乙胺-N-甲基转移酶(Pnmt)基因表达;运用Western blotting方法检测肾上腺低密度脂蛋白受体(LDLR)、B族I型清道夫受体(SR-BI)、StAR蛋白表达。结果 4种治法对氢化可的松造成的小鼠体质量下降和体温下降效果不显著,附子-肉桂组小鼠抓力显著高于模型组(P<0.05)。与模型组比较,知母-黄柏组小鼠血清皮质酮含量显著升高(P<0.01),StAR、Cyp11b1、Cyp11b2基因表达水平显著升高(P<0.05、0.01),SR-BI、StAR蛋白表达水平均显著升高(P<0.01)。结论滋阴泻火治法寒性药对(知母-黄柏)对小剂量氢化可的松诱发的药源性证候小鼠肾上腺皮质功能的纠正作用最佳。 Objective To study the effects of different Chinese materia medica combinations on the regulation of adrenal function.Methods Mice with 1.65 mg/(kg·d) hydrocortisone for 14 d to induce drug-induced syndrome was treated synchronously with assissting yang and dissipating cold(Aconm Lateralis Radix Praeparaia-Cinnamomi Cortex), nourishing yin and reducing fire(Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex), tonifying qi and promoting fluid production(Ginseng Radix et Rhizoma-Astragali Radix), and tonifying blood and benefiting spirit(Rehmanniae Radix Praeparata-Corni Fructus). The body mass of mice was dynamically observed every day, and the characteristic information such as body mass, holding power, axillary temperature,activity degrees of open field, and infrared temperature were detected by the experimental methodology of mice syndrome differentiation;The mice were sacrificed whose spleen, thymus was weighed and the organ index was calculated. Serum corticosterone,ACTH, adrenaline, and noradrenaline were measured by ELISA. The gene expressions of Star, Cyp11 a1, Cyp21 a1, Cyp11 b1,Cyp11 b2, Dbh, Ddc, Pnmt, and Th in the adrenal were detected by real-time fluorescent quantitative PCR. The protein’s expressions of LDLR, SRB1, and StAR were detected by Western blotting. Results The effects of four different treatments on the reduction of body weight and body temperature caused by hydrocortisone were not significant. The holding power of mice in Aconm Lateralis Radix Praeparaia-Cinnamomi Cortex group was significantly higher than that of hydrocortisone group(P < 0.05). Compared with the hydrocortisone group, the serum corticosterone content of mice in Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex group increased significantly(P < 0.01);The genes expression of Star, Cyp11 b1, and Cyp11 b2 was significantly up-regulated(P < 0.05, 0.01);The proteins expression of SRBI and StAR were significantly up-regulated. Conclusion The nourishing yin and purging fire treatment group(Anemarrhenae Rhizoma-Phellodendri C
作者 钱宏梁 潘志强 李亚 钱杨杨 费敏红 方肇勤 QIAN Hong-liang;PAN Zhi-qiang;LI Ya;QIAN Yang-yang;FEI Min-hong;FANG Zhao-qin(Basic Medical School of Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处 《中草药》 CAS CSCD 北大核心 2019年第10期2383-2389,共7页 Chinese Traditional and Herbal Drugs
基金 国家自然科学基金面上项目(81473562):国家自然科学基金面上项目(81873212).
关键词 药源性证候 氢化可的松 中药药对 肾上腺皮质 糖皮质激素 附子-肉桂 知母-黄柏 人参-黄芪 熟地-山茱萸 drug-induced syndrome hydrocortisone Chinese materia medica pair adrenal cortex glucocorticoid Aconm Lateralis Radix Praeparaia-Cinnamomi Cortex Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex Ginseng Radix et Rhizoma-Astragali Radix Rehmanniae Radix Praeparata-Corni Fructus
作者简介 钱宏梁(1992-),男,硕士研究生,主要从事中医证候实验研究;通信作者:潘志强(1977-),男,教授,博士,博士生导师,主要从事实验中医学教学与中医基础实验研究。Tel:(021)51322116,E-mail:pzq527@163.com.
  • 相关文献

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈