PCR technology is the major genetically modified(GM) crops detection method, but the purity and quality of the template is the major limitation, furthermore, it is not suitable for the detection of the sample that has low purity and quality. In this paper, we develop a novel detection method based on multiple displacement amplification.The limit of detection reaches to 10 copies. More over, the method have also detected certified reference material of GM maize Bt11 by single particle derived from maize powder. The result showed the detection rate of endogenous reference gene is up to 70%. The ratio of the detected event specific sequence to endogenous reference gene was in agreement with the certified values. The results provide a novel method for detection of traceable GMsamples,meanwhile, it also provides a potential solution for the detection of stacked GM crops.
Genomics and Applied Biology