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丁酸钠诱导不成熟树突状细胞分化过程中基因表达谱与微小RNA表达谱的研究

The expression profile of gene and microRNA during differentiation of immature dendritic cells induced by sodium butyrate
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摘要 目的利用基因芯片技术检测丁酸钠诱导人外周血来源的不成熟树突状细胞(DC)分化过程中基因表达谱和微小RNA(miRNA,miR)表达谱的变化,为进一步研究诱导DC分化的分子机制奠定基础。方法体外诱导人外周血单个核细胞来源的DC,经1mmol/L丁酸钠诱导分化24h,使用树突状和抗原递呈细胞基因芯片检测DC分化相关基因,并利用miRNA芯片技术检测、筛选显著表达差异的miRNA并进行靶基因预测,应用SPSS20.0统计软件分析,计量资料以均值±标准差(Mean±SD)表示,两组间比较采用t检验方法。结果丁酸钠诱导下DC共筛选出表达差异的基因有34个。上调的基因有14个,下调的基因有20个。改变的基因功能主要涉及细胞因子及其受体表达、抗原摄取、抗原递呈、细胞表面受体、及信号传导。丁酸钠诱导下DC表面标志CD83(8.90±6.63)显著低于对照组(18.11±3.34,t=3.037,P<0.05),表达差异的miRNA共34个,下调的miRNA基因有23个,上调的miRNA有11个。其中17个与组蛋白去乙酰化酶有关。结论丁酸钠诱导不成熟DC涉及多个基因的表达调控,伴随组蛋白去乙酰化酶及相关miRNA参与其中,共同控制并影响DC分化及功能改变。 Objective To investigate the expression profiles of gene and microRNA during the course of immature dendritic cells (DCs) differentiation induced by sodium butyrate in vitro. Methods The human monocyte-derived DCs were induced and total RNA of the cells was collected 24 h later. Gene array analysis of gene expression and microarray analysis of miRNA expression were performed on DCs and described with statistics. Results Thirty-four differentgenes were chosen by genearray analysis of DCs induced by sodium butyrate, including 14 up-regulated genes, and 20. Fourteen genes exhibited higher expressions, and 20 down-regulated genes as compared with control group. The function of these genes involved the secretion of the cytokines and the expression of their receptors, antigen uptake, antigen presentation, cell surface receptors, and signal transduction. The surface marker CD83 of DCs induced with sodium butyrate was (8.90±6.63), decreased significantly as compared with control group (t=3.037, P<0.05). Thirty-four different miRNAs were chosen by microarray analysis of DCs induced by sodium butyrate, including 23 miRNAs with higher expression, and 11 miRNAs with lower expression than the control group. Further analysis revealed that 17 miRNAs among the different miRNAs may keep close interaction with histone deacetylase. Conclusion During the course of immature DCs differentiation induced by sodium butyrate, multi-expression of the genes, accompanied with histone acetylase and related miRNAs, may play an important role in cell differentiation.
作者 褚自强 李琳 许鹤洋 来伟 曾育杰 刘璐 Chu Ziqiang;Li Lin;Xu Heyang;Lai Wei;Zeng Yujie;Liu Lu(Department of Gastrointestinal Surgery,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;Department of Gynaecology and Obstetrics,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China)
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2019年第6期1045-1047,共3页 Chinese Journal of Experimental Surgery
基金 国家自然青年科学基金(81703784) 广东省科技计划项目中医药联合科研专项(2017A020213028).
关键词 树突状细胞 丁酸钠 基因表达谱 微小RNA表达谱 Dendritic cell Sodium butyrate Gene expression profiles MicroRNA expression profiles
作者简介 通信作者:刘璐,Email:liulu23@mail.sysu.edu.cn.
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