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白术挥发油GC-MS指纹图谱与抗氧化活性的谱效关系研究

Study on the Spectrum-effect Relationship between GC-MS Fingerprint and Antioxidant Activity of Volatile Oil from Atractylodis Macrocephalae Rhizoma
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摘要 目的研究白术挥发油指纹图谱与抗氧化活性的谱效关系,筛选主要抗氧化活性成分。方法采用GC-MS测定不同产地的15批白术饮片挥发油指纹图谱,通过清除1,1-二苯基-2-苦腈基(DPPH)和Fe^3+还原/抗氧化能力法测定白术挥发油的抗氧化能力,运用偏最小二乘回归法进行数据分析,研究白术挥发油指纹图谱和抗氧化活性的相关性,并筛选活性色谱峰。结果 28个匹配指纹峰中,对挥发油清除DPPH自由基活性贡献度最大的前5个峰依次为:峰11>峰25>峰2>峰4>峰24;对挥发油还原Fe^3+能力贡献度最大的前5个峰依次为:峰11>峰8>峰14>峰19>峰26。其中峰11的峰面积与挥发油清除DPPH自由基能力和还原Fe^3+能力均呈正相关。通过与对照品比对确定峰11为苍术酮,并具有显著的抗氧化活性。结论苍术酮不仅是白术挥发油的主要成分,还是主要的抗氧化活性物质。 OBJECTIVE To study the spectrum-effect relationship between fingerprint and antioxidant activity of volatile oil from Atractylodis Macrocephalae Rhizoma, and to screen the main antioxidant active ingredients. METHODS GC-MS was used to determine the fingerprint of volatile oil from 15 batches of Atractylodis Macrocephalae Rhizoma from different producing areas. The antioxidant property of volatile oil was determined by removing 1,1-diphenyl-2-picrylonitrile(DPPH) and Fe^3+ reduction/anti-resistance method. The data were analyzed by partial least squares regression to study the correlation between the fingerprint and antioxidant activity of volatile oil. The active chromatographic peaks were then screened. RESULTS Among the 28 matching fingerprint peaks, the top 5 chromatographic peaks that significantly positively related to scavenge DPPH free radicals were as follow: peak 11>peak 25>peak 2>peak 4>peak 24. The top 5 chromatographic peaks that significantly positively related to restore Fe^3+ activity were as follow: peak 11>peak 8>peak 14>peak 19>peak 26. Peak area of peak 11 was positively correlated with both scavenge DPPH free radicals and restore Fe^3+ activity. By comparing with the reference substance, it was confirmed that the peak 11 was atractylon. The results showed that atractyrone had significant antioxidant activity. CONCLUSION Atractylon is not only the main component of Atractylodis Macrocephalae Rhizoma volatile oil, but also the main antioxidant active substance.
作者 许平翠 寿旦 吴人杰 李功华 王娜妮 XU Pingcui;SHOU Dan;WU Renjie;LI Gonghua;WANG Nani(Chinese Medicine Research Center, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China;College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China;Department of Pharmacy, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China)
出处 《中国现代应用药学》 CAS CSCD 北大核心 2019年第11期1322-1327,共6页 Chinese Journal of Modern Applied Pharmacy
基金 国家自然科学基金项目(81603252) 国家中药标准化项目(ZYBZH-Y-ZY-45) 浙江省自然科学基金项目(LQ17H280002) 浙江省中医药科技计划项目(2018ZY003,2016ZB008).
关键词 白术 挥发油 抗氧化活性 偏最小二乘回归法 谱效关系 Atractylodis Macrocephalae Rhizoma volatile oil antioxidant activity partial least squares regression spectral effect relationship
作者简介 许平翠,女,硕士生,Tel:(0571)88849089,E-mail:xpc123ok@163.com;通信作者:王娜妮,女,博士,副研究员,Tel:(0571)88849089,E-mail:wnn8511@163.com.
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