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西瓜枯萎病病原菌分离纯化及生物防治 预览

Isolation, Purification and Biological Control of Pathogen of Watermelon Fusarium Wilt
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摘要 目的:分离纯化并鉴定西瓜枯萎病病原菌,探索苦瓜籽蛋白粗提液对病原菌的防治作用。方法:利用平板划线法从西瓜枯萎病株中分离出枯萎病病原菌,采用病原菌孢子液对正常的西瓜幼苗进行盆栽转染,并观察苦瓜籽蛋白粗提液对西瓜枯萎病病原菌的抑制情况。结果:经鉴定西瓜枯萎病病原菌为尖孢镰刀菌。枯萎病菌孢子悬液可快速导致具有1~2片真叶的西瓜幼苗快速患上枯萎病。苦瓜籽蛋白粗提液浸泡的打孔滤纸出现抑菌圈。结论:西瓜枯萎病原菌为一种尖孢镰刀菌,苦瓜籽蛋白粗提液对西瓜枯萎病病原菌具有非常有效的抑菌作用。 Objective: The pathogen of watermelon fusarium wilt was isolated and purified. And the inhibition of the pathogen was investigated by adding the crude extract of bitter melon seed protein on the culture medium of the pathogen of Fusarium oxysporum. Methods: The pathogen of Fusarium oxysporum was isolated from the wilted watermelon plants by streaking plate method. Then the normal watermelon seedlings were infected with the spores of pathogen. Moreover, the inhibition of the crude extract of bitter melon seed protein on the pathogen of watermelon fusarium wilt was observed. Results: The colony of the pathogen of watermelon fusarium wilt, the form of the small/large conidia and the form of the mycelium are the same as that described by Buss, which is Fusarium oxysporum. The watermelon seedlings with one or two cotyledons could be easily infected by Fusarium oxysporum. On the medium with the pathogen, the perforated filter paper, which was soaked in the sterile water and the crude extract of bitter melon seed protein, was used for antagonistic experiment. Inhibition zone appeared in the experimental group while there was none in the control group. Conclusion: The pathogen for watermelon fusarium wilt was a kind of Fusarium oxysporum and the crude extract of bitter melon seed protein can effectively inhibit pathogenetic growth.
作者 谷富强 王袆玮 高春情 王雨停 陈心怡 刘京华 GU Fuqiang;WANG Yiwei;GAO Chunqing;WANG Yuting;CHEN Xinyi;LIU Jinghua(College of Life and Health Science, Anhui Science and Technology University, Fengyang 233100, China)
出处 《安徽科技学院学报》 2019年第2期29-33,共5页 Journal of Anhui Science and Technology University
基金 农业部水产品加工重点试验室开放基金(NYJG201802) 安徽科技学院大学生创新创业训练计划项目(2017X082) 安徽科技学院校级重点学科项目(AKZDXK2015B02)。
关键词 西瓜枯萎病 尖孢镰刀菌 苦瓜籽提取物 抑菌圈 Watermelon fusarium wilt Fusarium oxysporum Crude extract of bitter melon seeds Inhibition zone
作者简介 谷富强(1996-),男,安徽阜阳人,在读本科生,主要从事应用微生物学研究;通信作者:刘京华,讲师,E-mail:biology200801@163.com。
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