Objective To investigate the effects of Yiqi Yangyin Qingre Formula on glucagon-like peptide 2 (GLP-2), interferon-gamma (IFN-γ) and nuclear factor kappa B (NF-κB) in spontaneous type 2 diabetes (KKAy) mice. Methods A total of 50 male SPF KKAy mice were selected. They were randomly divided into a model group, a metformin group, as well as high, medium and low dose groups of Yiqi Yangyin Qingre Formula, with 10 mice in each group. And 10 C57BL/6J mice were taken as the normal group. Each group was given corresponding intervention. After 8 weeks of intervention, the levels of GLP-2, IFN-γ and NF-κB of mice were measured. Result Compared with the normal group, the levels of IFN-γ and NF-κB in the model group were increased significantly (P<0.01), and the GLP-2 level was decreased significantly (P<0.01). The IFN-γ level of KKAy mice could be decreased in the high, medium and low dose groups of Yiqi Yangyin Qingre Formula (P<0.05). The NF-κB level of mice could be decreased in the high dose group of Yiqi Yangyin Qingre Formula (P<0.05). And the GLP-2 level of mice could be increased in the high, medium and low dose groups of Yiqi Yangyin Qingre Formula (P<0.05). Conclusion Yiqi Yangyin Qingre Formula can reduce blood glucose and insulin resistance in diabetic mice, and its effects may be related to the increase of GLP-2 level and the reduction of inflammatory reaction.
Journal of Hunan University of Chinese Medicine
Yiqi Yangyin Qingre
glucagon-like peptide 2
nuclear factor-kappa B