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益气养阴清热方对KKAy小鼠GLP-2、IFN-γ、NF-κB的影响 预览

Effects of Yiqi Yangyin Qingre Formula on GLP-2, IFN-γ and NF-κB in KKAy Mice
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摘要 目的探讨益气养阴清热方对自发性2型糖尿病(KKAy)小鼠胰高血糖素样肽2(glucagon-likepeptide2,GLP-2)、γ干扰素(interferongamma,IFN-γ)、核因子κB(nuclearfactorkappabeta,NF-κB)的影响。方法取雄性SPF级KKAy小鼠50只,随机分为模型组,二甲双胍组,益气养阴清热方高、中、低剂量组,每组各10只;另取10只C57BL/6J小鼠作为正常组。各组予以相应干预。干预8周后检测各组小鼠GLP-2、IFN-γ、NF-κB水平。结果与正常组比较,模型组小鼠IFN-γ、NF-κB明显升高(P<0.01),GLP-2水平明显降低(P<0.01)。益气养阴清热方高、中、低剂量组均可降低KKAy小鼠IFN-γ水平(P<0.05);益气养阴清热方高剂量组可降低小鼠NF-κB水平(P<0.05);益气养阴清热方高、中、低剂量组均可升高小鼠GLP-2水平(P<0.05)。结论益气养阴清热方能改善糖尿病小鼠血糖、胰岛素抵抗,其作用可能与提高GLP-2水平、降低炎症反应相关。 Objective To investigate the effects of Yiqi Yangyin Qingre Formula on glucagon-like peptide 2 (GLP-2), interferon-gamma (IFN-γ) and nuclear factor kappa B (NF-κB) in spontaneous type 2 diabetes (KKAy) mice. Methods A total of 50 male SPF KKAy mice were selected. They were randomly divided into a model group, a metformin group, as well as high, medium and low dose groups of Yiqi Yangyin Qingre Formula, with 10 mice in each group. And 10 C57BL/6J mice were taken as the normal group. Each group was given corresponding intervention. After 8 weeks of intervention, the levels of GLP-2, IFN-γ and NF-κB of mice were measured. Result Compared with the normal group, the levels of IFN-γ and NF-κB in the model group were increased significantly (P<0.01), and the GLP-2 level was decreased significantly (P<0.01). The IFN-γ level of KKAy mice could be decreased in the high, medium and low dose groups of Yiqi Yangyin Qingre Formula (P<0.05). The NF-κB level of mice could be decreased in the high dose group of Yiqi Yangyin Qingre Formula (P<0.05). And the GLP-2 level of mice could be increased in the high, medium and low dose groups of Yiqi Yangyin Qingre Formula (P<0.05). Conclusion Yiqi Yangyin Qingre Formula can reduce blood glucose and insulin resistance in diabetic mice, and its effects may be related to the increase of GLP-2 level and the reduction of inflammatory reaction.
作者 蒋云霞 陈文辉 李双蕾 覃俏娜 陈薇宇 JIANG Yunxia;CHEN Wenhui;LI Shuanglei;QIN Qiaona;CHEN Weiyu(Endocrine Department, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, China;Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, China)
出处 《湖南中医药大学学报》 CAS 2019年第6期704-707,共4页 Journal of Hunan University of Chinese Medicine
基金 广西自然科学基金项目(2015GXNSFAA139119) 广西名老中医民族医传承工作室建设项目(桂卫中〔2014〕9号文件).
关键词 糖尿病 益气养阴清热 胰高血糖素样肽2 Γ干扰素 核因子ΚB diabetes mellitus Yiqi Yangyin Qingre glucagon-like peptide 2 interferon-gamma nuclear factor-kappa B
作者简介 蒋云霞,女,硕士,研究方向:中西医结合防治内分泌代谢性疾病;通讯作者:李双蕾,女,主任医师,教授,硕士研究生导师,E-mail:lslei66@126.com.
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