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薄荷素油鼻黏膜保湿微乳的制备及其黏膜黏附性和纤毛毒性研究 预览

Preparation of Peppermint Oil Moisturizing Microemulsion for Nasal Mucosa and Study on Its Mucosal Adhesion and Cilia Toxicity
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摘要 目的:制备薄荷素油鼻黏膜保湿微乳,并对其黏膜黏附性和纤毛毒性进行考察。方法:以聚氧乙烯氢化蓖麻油为乳化剂制备薄荷素油鼻黏膜保湿微乳,基于综合评分以正交设计法优化微乳的制备工艺;对所制微乳进行表征并采用气相色谱法测定薄荷醇含量;通过测定蟾蜍在体纤毛传输速率评价其黏膜黏附性,测定蟾蜍离体纤毛持续运动时间以评价其纤毛毒性。结果:自制微乳的优化制备工艺为先将薄荷素油与乳化剂分散,再加入无水乙醇、食用甘油、蒸馏水混合后,于1 200 r/min的转速下搅拌2h。3批自制微乳中薄荷醇的平均含量为2.682、2.507、2.496mg/mL,RSD为2.89%(n=3)。自制微乳高、中、低剂量组(以薄荷醇计2.561、0.256、0.128mg/mL)蟾蜍在体上颚纤毛传输速率分别为(0.65±0.01)、(0.78±0.03)、(0.92±0.04)cm/min,显著低于生理盐水组和复方薄荷脑滴鼻液组(P<0.05);蟾蜍离体上颚纤毛运动时间分别为(206.7±4.9)、(226.0±13.5)、(269.3±12.9)min,显著长于去氧胆酸钠组(P<0.05)。结论:自制微乳的制备工艺可行、质量可控,其黏膜黏附性较好,无纤毛毒性。 OBJECTIVE:To prepare the Peppermint oil moisturizing microemulsion for nasal mucosa and survey its mucosal adhesion and cilia toxicity.METHODS:The polyoxyethylene hydrogenated castor oil was used as emulsifier to prepare the Peppermint oil moisturizing microemulsion for nasal mucosa,and the preparation technology was optimized on the basis of comprehensive score by orthogonal design.The microemulsion was characterized and the menthol content was determined by GC.The mucosal adhesion was evaluated by measuring the transport rate by cilia in vivo,and the cilia toxicity of microemulsion was evaluated by measuring the sustained movement time of cilia in vitro.RESULTS:The optimal preparation technology of self-made microemulsion was to firstly disperse the peppermint oil and the emulsifier,then add anhydrous ethanol,edible glycerin and distilled water,and stir at 1 200 r/min for 2 h.The average contents of menthol in the three batches of the microemulsion were 2.682,2.507 and 2.496 mg/mL(RSD=2.89%,n=3),respectively.The cilia transport rates in vivo were(0.65±0.01),(0.78±0.03)and(0.92±0.04)cm/min in high-dose,medium-dose,and low-dose groups of self-made microemulsion(2.561,0.256,0.128 mg/mL of menthol)respectively,which were significantly lower than normal saline group and compound menthol nasal droups(P<0.05).The cilia movement time in vitro were(206.7±4.9),(226.0±13.5),(269.3±12.9)min,which were significantly longer than sodium deoxycholate group(P<0.05).CONCLUSIONS:The preparation technology of self-made microemulsion is easy-to-handle and controllable in quality.The prepared microemulsion shows good mucosal adhesion without cilia toxicity.
作者 荀波娜 毕青玲 谢茵 李萍 杨丽 毕小平 XUN Bona;BI Qingling;XIE Yin;LI Ping;YANG Li;BI Xiaoping(School of Pharmacy,Shanxi Medical University,Taiyuan 030001,China;Dept.of Otolaryngology,China-Japan Friendship Hospital,Beijing100029,China)
出处 《中国药房》 CAS 北大核心 2019年第12期1644-1649,共6页 China Pharmacy
基金 山西省科学技术厅应用基础研究项目(No.201801D221070).
关键词 薄荷素油 鼻黏膜 保湿 微乳 工艺优化 黏附性 纤毛毒性 Peppermint oil Nasal mucosa Moisturizing Microemulsion Technology optimization Adhesion Cilia toxicity
作者简介 荀波娜,硕士研究生。研究方向:高分子材料在药学中的应用。电话:0351-3985190。E-mail:791285117@qq.com;通信作者:毕小平,教授,硕士生导师。研究方向:高分子材料在药学中的应用。电话:0351-3985190。E-mail:bixping@163.com.
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