期刊文献+

低温条件微生物MICP沉淀产率试验研究

Experimental study on precipitation rate of MICP under low temperatures
分享 导出
摘要 低温导致微生物固化沉淀产率低,制约着该技术的应用。选取巨大芽孢杆菌,通过控制不同温度和pH值分析该菌种的生长繁殖特性和脲酶活性,并研究不同温度条件下的碳酸钙沉淀产率,通过采用营养液中添加尿素和低温驯化两种方法来提高低温条件下较低的沉淀产率,最后通过砂土固化试验,对比研究尿素添加方法和低温驯化对固化效果的影响。结果表明:温度越高,巨大芽孢杆菌的生长繁殖越快,脲酶活性越强,低温明显抑制其生长繁殖和脲酶活性;pH为8时,巨大芽孢杆菌生长繁殖最快,且脲酶活性最强;温度越高,沉淀产率越大;营养液中添加尿素和对巨大芽孢杆菌进行低温驯化都可以明显提高生长繁殖速度和沉淀产率,可以有效解决低温条件下碳酸钙沉淀不足问题,而将两者结合起来,沉淀产率提升更为明显;营养液中添加尿素和低温驯化都能提高砂土固化效果,而同时采用这两种方法固化效果提升更明显,该研究能有效解决低温条件沉淀少阻碍实际工程应用的问题,为后续低温条件微生物固化技术的应用打下基础。 The low deposition rate of microbial solidification technology in low temperatures often restricts its application. Bacillus megaterium is chosen, and by controlling the different temperatures and pH values, the growth characteristics and urease activities of such strain are analyzed, and the calcium carbonate precipitation yields under different temperature conditions are studied. By adding urea to nutrient solution and the domestication in low temperatures, the low precipitation rate is improved. Finally, the sand solidification tests are conducted to comparatively study the curing effect with adding urea to medium or the domestication of Bacillus megaterium in low temperatures. The results show that the higher the temperature, the faster the growth and reproduction of Bacillus megaterium and the stronger the urease activity. Low temperatures obviously inhibit its growth and urease activity. When pH is 8, the growth and reproduction of bacillus are the fastest, and the urease activity is the strongest. The higher the temperature, the higher the deposition rate. Adding urea to nutrient solution and the domestication of Bacillus megaterium in low temperatures both can obviously increase the speed of reproduction and precipitation yield, which can effectively solve the problem of lacking calcium carbonate precipitation at low temperatures. By combining the two methods, the increase in sediment yields is more obvious. Adding urea to nutrient solution and the domestication of Bacillus megaterium in low temperatures both can improve the effect of soil solidification, and at the same time, using the two methods together, the curing effect promotion is more obvious. Therefore, the study can effectively solve the problem that less precipitation at low temperatures will obstacle actual engineering application, and lay a solid foundation for the subsequent application of MICP technology at low temperatures.
作者 孙潇昊 缪林昌 吴林玉 王呈呈 陈润发 SUN Xiao-hao;MIAO Lin-chang;WU Lin-yu;WANG Cheng-cheng;CHEN Run-fa(Institute of Geotechnical Engineering,Southeast University,Nanjing 210096,China)
出处 《岩土工程学报》 EI CAS CSCD 北大核心 2019年第6期1133-1138,共6页 Chinese Journal of Geotechnical Engineering
基金 国家自然科学基金项目(51578147) 东南大学优秀博士学位论文培育基金项目(YBJJ1846).
关键词 低温 巨大芽孢杆菌 沉淀产率 尿素 驯化 砂土固化 low temperature Bacillus megaterium precipitation rate urea domestication sand solidification
作者简介 孙潇昊(1993-),男,博士,主要从事隧道地铁的研究工作。E-mail:sunxiao14hao@126.com.
  • 相关文献

参考文献6

二级参考文献76

共引文献185

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈