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高效液相色谱法测定人工牛黄甲硝唑胶囊中胆红素含量 预览

Content Determination of Bilirubin in Galculus Bovis and Metronidazole Capsules by HPLC
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摘要 目的建立测定人工牛黄甲硝唑胶囊中胆红素含量高效液相色谱(HPLC)法。方法色谱柱采用Agilent ZORBAX SB-C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-1%冰醋酸溶液(95∶5),检测波长为453 nm,柱温为30℃,流速为1.0 mL/min,进样量为5μL。结果胆红素质量浓度在0.442~11.05μg/m L(r=0.999 9)范围内与峰面积线性关系良好;平均回收率为99.45%,RSD为1.02%(n=9)。结论该法简便、精密度好、结果准确,可用于人工牛黄甲硝唑胶囊中胆红素的含量测定。 Objective To establish an HPLC method for the content determination of bilirubin in Galculus Bovis and Metronidazole Capsules.Methods The chromatographic column was Agilent ZORBAX SB-C18 column(150 mm×4.6 mm,5μm),the mobile phase was acetonitrile-1%acetic acid(95∶5),the detection wavelength was 453 nm,the column temperature was 30℃,the flow rate was1.0 mL/min and the sample size was 5μL.Results The bilirubin showed good linear relationship with peak area in the range of0.442-11.05μg/m L(r=0.999 9),the average recovery was 99.45%and RSD was 1.02%(n=9).Conclusion The method is simple,accurate with good reproducibility,which can be used for the content determination of bilirubin in Galculus Bovis and Metronidazole Capsules.
作者 李香荷 王强 高燕霞 张冬 郭毅 张轶华 LI Xianghe;WANG Qiang;GAO Yanxia;ZHANG Dong;GUO Yi;ZHANG Yihua(Hebei InsMute for Drug Control,Shijiazhuang,Hebei,China 050011)
出处 《中国药业》 CAS 2019年第13期39-41,共3页 China Pharmaceuticals
关键词 人工牛黄甲硝唑胶囊 胆红素 高效液相色谱法 Galculus Bovis and Metronidazole Capsules bilirubin HPLC
作者简介 第一作者:李香荷,女,硕士研究生,主管药师,研究方向为抗生素药品检验,(电子信箱)125186584@qq.com。
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