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苦蘵内生真菌Trichoderma harzianum的次级代谢产物

Secondary Metabolites of Endophytic Fungus Trichoderma harzianum Isolated from Physalis angulata
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摘要 目的:研究茄科酸浆属植物苦蘵(Physalis angulata)的内生真菌哈茨木霉(Trichoderma harzianum)的次级代谢产物。方法:菌株采用震荡培养箱扩大培养,得到的菌液用乙酸乙酯等体积萃取3次,菌丝体则以丙酮浸泡超声,回收溶剂后将所得提取物合并,再经液相-质谱联用技术,开放ODS反相柱色谱,LH-20羟丙基葡聚糖凝胶柱色谱(sephadex LH-20),高效液相色谱等方法进行成分的分析与分离纯化活性次级代谢产物。利用其理化性质和光谱数据(1H-NMR,13C-NMR)等鉴定化合物结构。采用噻唑蓝(MTT)法测定化合物的细胞毒性。结果:从苦蘵内生真菌哈茨木霉中分离得到10个次级代谢产物,分别鉴定为destruxin A2(1),destruxin B2(2),环(亮-脯)二肽(3),环(苯丙-脯)二肽(4),cyclonerodiol(5),brevianamide F(6),N-乙酰色胺(7),9-羟基-邻苯二甲酸二异丁基酯(8),5-羟基-3-羟甲基-2-甲基-7-甲氧基色酮(9)和苯丙酸(10)。细胞毒性试验结果显示,化合物1~10对肺癌细胞株A549均未表现出显著的细胞毒性(半抑制浓度≥20 mg·L^-1)。结论:化合物1~10均首次从哈茨木霉中分离得到。 Objective: To study the secondary metabolites of the endophytic fungus Trichoderma harzianum,which was isolated from Physalis angulata. Method: The strains were cultured in a big-scale by oscillating incubator. Then the obtained culture liquid and mycelia were extracted by EtOAC and actone, respectively. The extracts were integrated after recovery of solvents,and then the active secondary metabolites were isolated and purified by comprehensive use of open ODS flash column,sephadex LH-20,HPLC,and LC-MS analysis techniques. Their structures were identified according to their physico-chemical properties and nuclear magnetic resonance ( NMR) data. The cytotoxicities of the compounds were determined by methyl thiazolyl tetrazolium ( MTT) assay. Result: Ten compounds isolated from T. harzianum were identified as destruxin A2 ( 1),destruxin B2 ( 2),3-isobutyl-pyrrolopiperazine-2,5-dione [cyclo ( leu-pro dipeptide)]( 3 ),cyclo ( Phen-pro) dipeptide ( 4 ),cyclonerodiol ( 5 ),brevianamide F ( 6 ),N-acetyltryptamine ( 7 ),9-hydroxyl-( 2-methylpropyl) isobutyl phthalate ( 8 ),5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone ( 9 ),and phenylpropionic acid ( 10). Compounds 1-10 didn't exhibit significant cytotoxicity to lung cancer cell line A549 in the MTT assay ( IC50≥20 mg·L ^- 1 ). Conclusion: All compounds were isolated from T. harzianum for the first time.
作者 唐梦月 樊佳佳 刘霞 刘志国 裴月湖 孙奕 TANG Meng-yue;FAN Jia-jia;LIU Xia;LIU Zhi-guo;PEI Yue-hu;SUN Yi(Shenyang Pharmaceutical University,Shenyang 110016,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Chemistry,Chemical Engineering and Life Science,Wuhan University ofTechnology,Wuhan 430070,China)
出处 《中国实验方剂学杂志》 CAS CSCD 北大核心 2019年第14期186-191,共6页 China Journal of Experimental Traditional Medical Formulae
基金 国家自然科学基金项目(81502968) 中国中医科学院自主选题研究项目(ZZ0908057).
关键词 苦蘵 内生真菌 哈茨木霉 次级代谢产物 细胞毒性 Physalis angulata endophytic fungus Trichoderma harzianum secondary metabolites cytotoxicity
作者简介 第一作者:唐梦月,在读硕士,从事天然产物活性成分研究,E-mail:1525122603@qq.com;通信作者:裴月湖,博士,教授,从事天然药物研究,E-mail:peiyueh@vip.163.com;通信作者:孙奕,博士,副研究员,从事天然产物活性成分研究,E-mail:ysun@icmm.ac.cn.
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