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五灵脂的质量标准研究 预览

Study on the quality standard of Faeces trogopterori
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摘要 目的建立五灵脂的3种药用成分薄层色谱鉴别(TLC)和含量测定方法。方法采用TLC法定性鉴别五灵脂中的槲皮素、山柰素和穗花杉双黄酮;采用HPLC法测定五灵脂中槲皮素、山柰素和穗花杉双黄酮的含量。结果五灵脂中槲皮素、山柰素和穗花杉双黄酮的TLC图斑点清晰、重复性好、专属性强、分离较好、方法简便。药材中槲皮素、山柰素和穗花杉双黄酮的线性范围分别为0.11~1.08(r1=0.9998),0.38~4.78(r2=0.9996)和0.45~4.46μg(r3=0.9998),平均加样回收率分别为99.01%,99.13%和98.73%,RSD值分别为0.65%,0.34%和0.48%;3个指标测定的精密度、重复性、稳定性和准确度实验RSD值均低于2.00%。结论该方法简便、快捷,结果准确、可靠,可作为该药材的质量控制标准。 Objective To establish the methods of TLC identification and content determination of 3 medicinal components in Faeces trogopterori.Methods Quercetin,kaempferol and amentoflavone in Faeces trogopterori were identified qualitatively by TLC,and the contents of quercetin,kaempferol and amentoflavone in Faeces trogopterori were determined by HPLC.Results The TLC of quercetin,kaempferol and amentoflavone in Faeces trogopterori showed clear spots,good repeatability,strong specificity,and good separation.Quercetin showed a good linear relationship in the range of 0.11-1.08μg(r1=0.999 8)with an average recovery of 99.01%(RSD was 0.65%).Kaempferide showed a good linear relationship in the range of 0.38-4.78μg(r2=0.999 6)with an average recovery of 99.13%(RSD was 0.34%).Amentoflavone showed a good linear relationship in the range of 0.45-4.46μg(r3=0.999 8)with an average recovery of 98.73%(RSD was 0.48%).The RSDs of precision,repeatability,stability and accuracy of the 3 indicators were all below 2.00%.Conclusion The method is simple,fast,accurate and reliable,and can be used as the quality control method of this medicinal materials.
作者 陈雪琴 贾文江 周仔莉 李宏 南沣 佘龙 CHEN Xueqin;JIA Wenjiang;ZHOU Zili;LI Hong;NAN Feng;SHE Long(Shangluo Drug Inspection Institute,Shangluo 726000,China)
出处 《西北药学杂志》 CAS 2019年第4期469-473,共5页 Northwest Pharmaceutical Journal
关键词 五灵脂 TLC法 HPLC法 质量标准 Faeces trogopterori TLC HPLC quality standard
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