目的观察外周血4种细胞因子(IL-2、IL-4、IL-15及IL-21)在HIV感染者/AIDS患者(HIV/AIDS)和正常人表达水平的区别及其与HIV RNA病毒载量和CD3+、CD4+细胞数量的相关性,分析其与HIV感染的关系和临床意义。方法40例未经抗病毒治疗的HIV/AIDS和31名正常人,采用ELISA法检测外周血4种细胞因子(IL-2、IL-4、IL-15及IL-21),使用流式细胞计数法检测外周血CD3+、CD4+T淋巴细胞数量,实时聚合酶链反应测定血清中HIV RNA水平。结果未经抗病毒治疗的HIV/AIDS外周血IL-2、IL-15及IL-21水平低于正常人,而IL-4水平高于正常人(P<0.05)。CD3+、CD4+细胞数量和各细胞因子水平显示出一定的相关关系,IL-2和IL-15水平均与HIV-1 RNA载量呈负相关。结论提示外周血4种细胞因子都可能参与了HIV感染的致病机制,并与HIV RNA病毒载量及CD3+、CD4+细胞数量相关。
Objective To observe the differences of 4 cytokine levels between HIV/AIDS patients and normal persons, and their relevance with HIV RNA level and CD3+, CD4+ cell count, and to analyze the relationship with HIV infection and clinical significance. Methods A total of 40 HIV/AIDS patients without antiviral therapy and 31 normal persons were enrolled. Enzyme Linked Immunosorbent Assay (ELISA) was used to detect 4 cytokine levels (IL-2, IL-4, IL-15 and IL-21). At the same time, the number of CD3+ and CD4+ T lymphocytes in peripheral blood was detected by flow cytometry, and the level of HIV RNA in serum was determined by real-time polymerase chain reaction. Results IL-2, IL-15 and IL-21 in peripheral blood of HIV/AIDS patients without antiviral therapy were significantly lower than that of the normal people, and IL-4 level was higher than the normal people(P<0.05). The number of CD3+, CD4+ cells and the level of each cytokine showed a certain correlation, and IL-2 and IL-15 levels were negatively correlated with HIV-1 RNA load. Conclusion It is suggested that all 4 cytokines in peripheral blood may be involved in the pathogenesis of HIV infection and are related to HIV RNA viral load and the number of CD3+ and CD4+ cells.
Journal of Hunan University of Chinese Medicine