期刊文献+

HIV/AIDS患者外周血中4种细胞因子IL-2、IL-4、IL-15和IL-21表达水平及其临床意义研究 预览

Four Cytokine Expression Levels of IL-2, IL-4, IL-15 and IL-21 in Peripheral Blood of HIV/AIDS Patients and Their Clinical Significance
在线阅读 下载PDF
分享 导出
摘要 目的观察外周血4种细胞因子(IL-2、IL-4、IL-15及IL-21)在HIV感染者/AIDS患者(HIV/AIDS)和正常人表达水平的区别及其与HIV RNA病毒载量和CD3+、CD4+细胞数量的相关性,分析其与HIV感染的关系和临床意义。方法40例未经抗病毒治疗的HIV/AIDS和31名正常人,采用ELISA法检测外周血4种细胞因子(IL-2、IL-4、IL-15及IL-21),使用流式细胞计数法检测外周血CD3+、CD4+T淋巴细胞数量,实时聚合酶链反应测定血清中HIV RNA水平。结果未经抗病毒治疗的HIV/AIDS外周血IL-2、IL-15及IL-21水平低于正常人,而IL-4水平高于正常人(P<0.05)。CD3+、CD4+细胞数量和各细胞因子水平显示出一定的相关关系,IL-2和IL-15水平均与HIV-1 RNA载量呈负相关。结论提示外周血4种细胞因子都可能参与了HIV感染的致病机制,并与HIV RNA病毒载量及CD3+、CD4+细胞数量相关。 Objective To observe the differences of 4 cytokine levels between HIV/AIDS patients and normal persons, and their relevance with HIV RNA level and CD3+, CD4+ cell count, and to analyze the relationship with HIV infection and clinical significance. Methods A total of 40 HIV/AIDS patients without antiviral therapy and 31 normal persons were enrolled. Enzyme Linked Immunosorbent Assay (ELISA) was used to detect 4 cytokine levels (IL-2, IL-4, IL-15 and IL-21). At the same time, the number of CD3+ and CD4+ T lymphocytes in peripheral blood was detected by flow cytometry, and the level of HIV RNA in serum was determined by real-time polymerase chain reaction. Results IL-2, IL-15 and IL-21 in peripheral blood of HIV/AIDS patients without antiviral therapy were significantly lower than that of the normal people, and IL-4 level was higher than the normal people(P<0.05). The number of CD3+, CD4+ cells and the level of each cytokine showed a certain correlation, and IL-2 and IL-15 levels were negatively correlated with HIV-1 RNA load. Conclusion It is suggested that all 4 cytokines in peripheral blood may be involved in the pathogenesis of HIV infection and are related to HIV RNA viral load and the number of CD3+ and CD4+ cells.
作者 刘琼华 刘志生 郑煜煌 陈霞 贺波 郑力文 LIU Qionhua;LIU Zhisheng;ZHENG Yuhuang;CHEN Xia;HE Bo;ZHENG Liwen(Department of Medicine, Loudi Medical School, Loudi, Hunan 420000, China;Department of Infectious Diseases, Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China;Clinical Laboratory, Xiangya Hospital, Central-South University, Changsha, Hunan 410018, China;Department of Orthopedics, Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China)
出处 《湖南中医药大学学报》 CAS 2019年第8期1011-1014,共4页 Journal of Hunan University of Chinese Medicine
关键词 人免疫缺陷病毒 艾滋病 IL-2 IL-4 IL-15 IL-21 human immunodeficiency virus AIDS IL-2 IL-4 IL-15 IL-21
作者简介 刘琼华,女,讲师,研究方向:传染性疾病的防治,E-mail:578941158@qq.com.
  • 相关文献

参考文献4

二级参考文献40

  • 1郑煜煌,陈军,雷建华,赖力英,朱映华,何艳,罗虹雨,周华英,丁佩佩,张春迎,李慧.高效抗逆转录病毒疗法治疗HIV-1感染者的免疫学变化[J].中华皮肤科杂志,2006,39(5):281-284. 被引量:21
  • 2Jacobson MA,Zegans M,Pavan PR,et al.Cytomegalovirus retinitis after initiation of HAART.Lancet,1997,349 (9063):1443-1445. 被引量:1
  • 3International Network for the Study of HIV-associated IRIS (IN-SHI) http://www.inshi.umn.edu/definitions/General IRIS/home.html. 被引量:1
  • 4French MA,Price P,Stone SF.Immune restoration disease after antiretroviral therapy.AIDS,2004,18(12):1615-1627. 被引量:1
  • 5Shelburne SA,Montes M,Hamill RJ.Immune reconstitution inflammatory syndrome:more answers,more questions.J Antimicrob Chemother,2006,57(2):167-170. 被引量:1
  • 6Battegay M,Nuesch R,Rirschel B,et al.Immunological recovery and antiretroviral therapy in HIV-1 infection.Lancet Infect Dis,2006,6(5):280-287. 被引量:1
  • 7Hua-ying Zhou,Yu-huang Zheng,Yan He,et al.Evaluation of a 6-year hightly active antiretroviral therapy in Chinese HIV-1 infecyed patients.Intervirology,2010,53 (3):240-246. 被引量:1
  • 8Iyasere C,Tilton JC,Johnson AJ,et al.Diminished proliferation of human immunodeficiency virus-specific CD4 + T cells is associated with diminished interleukin-2 (IL-2) production and is recovered by exogenous IL-2.J Virol,2003,77(20):10900-10909. 被引量:1
  • 9Fackoya A,Matear PM,Filley E,et al.HIV infection alters the production of both type 1 and type 2 cytokines but does not induce a polarized type 1 or2 state.AIDS,1997,11 (12):1445-1452. 被引量:1
  • 10Zhou HY, Zheng YH, He Y, et al. Evaluation of a 6-year highly active antiretroviral therapy in Chinese HIV-1-infected patients. Intercirology, 2010, 53(4): 240-246. 被引量:1

共引文献27

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈