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西瓜枯萎病病原菌鉴定及药剂联合增效作用 预览

Pathogen identification of watermelon Fusarium oxysporum and its synergistic effect of fungicides
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摘要 采用PDA培养基和PD培养基,对西瓜病株病原菌进行分离、培养,经致病性测定,形态学及r DNA-ITS测序,鉴定其致病菌为尖孢镰刀菌(Fusarium oxysporum)。采用菌丝生长速率法测定85%氨基寡糖素和97%咪鲜胺对西瓜尖孢镰孢菌的共毒系数。结果显示,85%氨基寡糖素∶97%咪鲜胺=3∶1时,增效作用最好,共毒系数为167.88,对西瓜枯萎病菌菌丝具有很好的抑制效果。 Pathogen of watermenlon wilt disease was isolated and cultured on PDA and PD,which was indentified was Fusarium oxysporum Schlecht by analysis of morphological characteristics and rDNA-ITS sequence and pathogenic determination.The co-toxicity coefficient of 85% oligosaccharins and 97% prochloraz mixed against Fusarium oxysporum were tested in lab by mycelium growth rate method,respectively.The results showed that synergistic inhibition best when 85% oligosaccharins and 97% prochloraz combinations mixed by 3∶1,co-toxicity coefficient was 167.88,obviously inhibitory against the pathogen.
作者 王开成 WANG Kai-cheng(Agricultural Service Center of Huangtong Town of Lingao County,Lingao 571823,Hainan,China)
出处 《湖北农业科学》 2019年第14期59-61,82共4页 Hubei Agricultural Sciences
关键词 西瓜枯萎病菌(Fusarium oxysporum) 杀菌剂 联合作用 混配 Fusarium oxysporum fungicides combined action mixture
作者简介 王开成(1962-),男,海南临高人,农艺师,主要从事农业新技术研究与推广,(电话)13617573390(电子信箱)island62@126.com。
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