从南瓜籽油中提取的南瓜籽甾醇用于对SD大鼠体内抗氧化作用影响的研究。实验中将南瓜籽甾醇分为80、160、320 mg/(kg·d) 3种剂量灌胃SD大鼠,以不灌胃南瓜籽甾醇的SD大鼠为空白对照组。35 d后处死全部SD大鼠并取其血清和肝脏测定总抗氧化能力(T-AOC)水平、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性、丙二醛(MDA)和血清中8-羟基脱氧鸟苷(8-OHdG)含量。结果表明:与空白对照组相比,3种剂量组SD大鼠血清和肝脏中的TAOC水平、GSH-Px和SOD活性升高,而血清和肝脏中MDA以及血清中8-OHd G含量降低。说明南瓜籽甾醇对SD大鼠体内抗氧化能力的增强具有一定的促进作用,并且这种作用存在一定的剂量依赖性。
Pumpkin seed sterol extracted from pumpkin seed oil was used to study its effect on antioxidant activity in SD rats. In the experiment,pumpkin seed sterol was divided into three doses of 80,160,320 mg/(kg·d) to feed SD rats,and SD rats without gavage of pumpkin seed sterol were used as blank control group. After 35 d,all SD rats were sacrificed,and their serum and liver were taken for determination of total antioxidant capacity (T-AOC) level,glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities,malondialdehyde (MDA) and serum 8-hydroxydeoxyguanosine (8-OHdG)contents. The results showed that T-AOC levels,GSH-Px and SOD activities increased in serum and liver of SD rats in the three dose groups compared with the blank control group. MDA content in serum and liver and 8-OHdG content in serum decreased. It indicated that pumpkin seed sterol could promote the enhancement of antioxidant capacity in SD rats,and this effect was dose-dependent to some extent.
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