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大麦全基因组WRKY基因家族生物信息学分析

Bioinformatics Analysis of WRKY Gene Family in Barley Genome
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摘要 WRKY基因家族是一类含有WRKY保守结构域的转录因子,参与植物众多生理生化过程,在植物生长发育、代谢以及胁迫应答等过程中发挥重要作用。目前,已在多种植物中对WRKY转录因子进行了系统鉴定和分析,但是还未见有大麦WRKY基因家族系统筛选和鉴定的报道。本研究利用最新的大麦基因组数据,经生物信息学鉴定共得到98条大麦WRKY转录因子,其编码蛋白质序列长度为117~676 aa。有91条可以定位到大麦7条染色体上并呈不均匀分布,3H染色体上分布最多达25条,6H上最少仅有5条。通过系统进化分析,大麦98个WRKY转录因子根据其保守结构域可分为Ⅰ、Ⅱ和Ⅲ组,各组分别有10个、52个和36个成员,其中Ⅱ组可进一步分成Ⅱ-a、Ⅱ-b、Ⅱ-c、Ⅱ-d和Ⅱ-e 5个亚组。保守基序分析表明,大麦的20个保守基序中,有6个为WRKY结构域的保守基序,各组内部成员拥有一致的保守基序。本研究可为进一步开展大麦WRKY基因的发掘与功能验证提供科学依据。 WRKY is a superfamily of transcription factor,containing a conserved WRKY domain.The WRKY families plays important roles in plant by regulating various physiological and biochemical processes such as development,metabolism and response to stress.WRKY genes have been identified with systematic analysis and comparison in various plants.However,little information has been reported about the systematic identification and analysis of WRKY genes in barley.In the present study,we identified 98 WRKY genes in barley with bioinformatics information using the barley genome data,and the protein length ranged from 117 to 676 aa.Location analysis revealed that 91 WRKY genes with specific location information were distributed unevenly on all 7 chromosomes.Most of the WRKYs were presented on chromosome 3 H(25),while the fewest on chromosome 6 H(5).According to the structure of conserved domain,all together 98 WRKYs were classified into three major groups,including 10 type-Ⅰgenes,52 type-Ⅱgenes and 36 type-Ⅲgenes.Furthermore,type-Ⅱgenes can be divided into 5 groups,namelyⅡ-a(5),Ⅱ-b(4),Ⅱ-c(21),Ⅱ-d(7)andⅡ-e(15).MEME analysis showed that among the 20 conserved motifs in barley,6 were affirmed in WRKY domains,and similar conserved motifs were shared within groups.This study provides a foundation for function and evolution analysis of WRKY genes in barley.
作者 毛成志 王蕾 孔豆豆 王寒冬 张怀刚 沈裕虎 徐金青 Mao Chengzhi;Wang Lei;Kong Doudou;Wang Handong;Zhang Huaigang;Shen Yuhu;Xu Jin-qing(Key Laboratory of Adaptation and Evolution of Plateau Biota(AEPB),Qinghai Provincial Key Laboratory of Crop Molecular Breeding,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810001;University of Chinese Academy of Sciences,Beijing,100049)
出处 《分子植物育种》 CAS CSCD 北大核心 2019年第23期7623-7633,共11页 Molecular Plant Breeding
基金 青海省科技成果转化专项(2016-NK-131) 青海省创新平台建设专项项目(2017-ZJ-Y14) 中科院科技服务网络(STS)计划区域重点项目“青海高寒农牧交错区饲草品种培育与推广”共同资助。
关键词 大麦(Hordeum vulgare L.) 转录因子 WRKY系统 进化分析 Barley(Hordeum vulgare L.) Transcriptional factor WRKY Phylogenetic analysis
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