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油菜质膜水孔蛋白BnPIP1基因启动子区的克隆及初步的功能分析 被引量:6

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摘要 利用双链接头介导PCR的染色体步行技术,克隆了油菜质膜水孔蛋白BnPIP1基因上游1.6kb的调控区域(GenBank登录号为AF472487)。序列分析表明,该片段中含有种子萌发特异性序列及维管束特异性序列。将其全长片段及5'端不同长度的缺失片段与ugs(uidA)基因连接构建植物表达载体,转化烟草。GUS组织化学染色表明,全长1.6kb片段具有较强的启动子活性。GUS染色主要分布在细胞迅速增生的部位及维管束组织中。启动子缺失试验的GUS染色结果表明,-1610-1030bp区段的缺失使gus基因的表达明显变弱,推测该区段含有启动子的正调控元件;-1030-902bp可能存在强烈抑制基因表达的负调控元件;-902-19bp的片段亦可驱动gus基因的高水平表达。
出处 《中国科学:C辑》 CSCD 北大核心 2002年第6期519-526,T001共9页 Science in China(Series C)
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参考文献25

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二级参考文献3

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