目的观察卒中后抑郁(post-stroke depression, PSD)大鼠外周血及海马中IL-1β的表达,探讨IL-1β在PSD发病中的作用。方法 250-300g雄性SD大鼠,分为对照组、PSD组。PSD组分三个阶段连续监测:缺血性脑卒中(Ischemic stroke,IS)再灌注24h后、PSD造模2周末、PSD造模4周末。预饲养一周后线栓法制备IS模型,恢复一周后给予大鼠温和不可预知性刺激,持续4周,制备PSD模型。分别用ELISA法和免疫组化法检测血清和海马中IL-1β的表达。结果与对照组相比,IS大鼠血清中IL-1β表达增高,PSD组造模2周末、PSD组造模4周末时持续增高。与对照组相比,IS大鼠海马中IL-1β显著升高,24h达高峰,在PSD造模2周末和PSD造模4周末时,海马内IL-1β的表达相对于IS降低,但依然高于正常对照组。结论 IL-1β可能参与PSD发病的病理过程。
Objective To observe the expression of IL-1β in serum and hippocampus of post-stroke depression rats, and explore the role of IL-1β in the pathogenesis of post-stroke depression(PSD). Methods Male SD rats with 250-300 g were divided into control group and PSD group. The PSD component was continuously monitored in three stages: ischemic stroke(IS) 24 hours after reperfusion, 2 weeks of PSD modeling, and 4 weeks of PSD modeling. One week after pre-feeding, IS model was prepared by suture method. After one week of recovery, the rats were given mild unpredictable stimulation for 4 weeks to prepare the PSD model. The expression of IL-1β in serum and hippocampus was detected by ELISA and immunohistochemistry, respectively. Results Compared with the control group, the expression level of IL-1β in the serum of IS rats increased, continued to be increased in PSD groups at the end of 2 weeks and 4 weeks. The expression level of IL-1β in hippocampus was significantly elevated in IS rats than in control rats, reaching a peak at 24 hours, but lower in PSD group than in IS rats, but still higher than in control group. Conclusion IL-1β might be involved in the pathological process of PSD.
Progress of Anatomical Sciences