期刊文献+

曲古抑菌素A通过上调原钙黏蛋白9表达抑制人胃癌SGC-7901细胞迁移和侵袭

Tichostatin A inhibiting migration and invasion of human gastric carcinoma SGC-7901 cells via up-regulating protocadherin 9
收藏 分享 导出
摘要 目的探讨曲古抑菌素A(TSA)对人胃癌SGC-7901细胞迁移和侵袭能力的影响,及其可能的分子机制。方法体外常规培养SGC-7901细胞至对数生长期,给予不同浓度TSA(5、10、20、40、80、160 nmol/L)处理48 h,采用细胞计数试剂盒(CCK-8)法检测细胞活力,脂质体介导法将人原钙黏蛋白9(PCDH9)真核表达质粒转染SGC-7901细胞并筛选出稳定表达的细胞系,Transwell实验检测迁移率和侵袭率,RT-PCR法检测PCDH9 mRNA表达水平,免疫印迹法(Western blotting)检测PCDH9、Snail、E-钙黏蛋白(E-cadherin)、基质金属蛋白酶2(MMP-2)和MMP-9的蛋白表达水平。结果 TSA在80 nmol/L以上时对人胃癌SGC-7901细胞活力有显著的抑制作用(P<0.05);而在较低浓度下(5~20 nmol/L)可剂量依赖性地抑制人胃癌SGC-7901细胞的迁移和侵袭能力(P<0.05),下调Snail、MMP-2和MMP-9蛋白表达水平(P<0.05),上调E-cadherin和PCDH9蛋白表达水平(P<0.05),上调PCDH9 mRNA表达水平(P<0.05);PCDH9高表达也可抑制SGC-7901细胞迁移和侵袭(P<0.05),上调E-cadherin蛋白表达并下调Snail、MMP-2和MMP-9蛋白表达(P<0.05)。结论 TSA可在体外抑制胃癌SGC-7901细胞迁移和侵袭,这可能与其促进PCDH9基因表达,继而下调Snail、MMP-2和MMP-9蛋白表达,上调E-cadherin蛋白表达有关。 Objective To investigate the effect of trichostatin A(TSA)on migration and invasion in human gastric carcinoma SGC-7901 cells and its possible mechanism.Methods SGC-7901 cells were cultured in vitro and treated with TSA(5,10,20,40,80,160 nmol/L)for 48 hours,and then the cell viability was detected by cell counting kit 8(CCK-8)assay.The protocadherin 9(PCDH9)high-expression SGC-7901 cells were stably established by transfecting with eukaryotic expression vector(pCMV6-PCDH9).Transwell assay was used to determine the abilities of migration and invasion.The mRNA expression level of PCDH9 were measured by RT-PCR.Western blotting was performed to analyze the protein expression of PCDH9,Snail,E-cadherin,matrix metalloproteinase(MMP)-2 and MMP-9.Results TSA remarkably reduced the cell viability of SGC-7901 cells in excess of 80 nmol/L(P<0.05).However,in a dose-dependent manner,low-level TSA(5-20 nmol/L)suppressed migration and invasion of SGC-7901 cells(P<0.05),down-regulated the protein levels of Snail,MMP-2 and MMP-9(P<0.05),and up-regulated the protein levels of PCDH9 and E-cadherin(P<0.05).Meanwhile,high expression of PCDH9 also inhibited migration and invasion of SGC-7901 cells(P<0.05),down-regulated the protein levels of Snail,MMP-2 and MMP-9(P<0.05),and up-regulated the protein level of E-cadherin(P<0.05).Conclusion TSA may inhibit migration and invasion of SGC-7901 cells most likely via up-regulating PCDH9,and then down-regulating the protein levels of Snail,MMP-2 and MMP-9,and up-regulating the protein level of E-cadherin.
作者 程琦 刘晓东 徐宛玲 CHENG Qi;LIU Xiao-dong;XU Wan-ling(Department of Pathology,Luohe Medical College,He’nan Luohe 462002,China;Department of Anatomy,Luohe Medical College,He’nan Luohe 462002,China;Department of Internal Medicine,Luohe Medical College,He’nan Luohe 462002,China)
出处 《解剖学报》 CAS CSCD 北大核心 2019年第5期595-600,共6页 Acta Anatomica Sinica
基金 河南省科技厅科技发展计划项目(132300410315).
关键词 曲古抑菌素A 原钙黏蛋白9 胃癌 SGC-7901细胞 免疫印迹法 Trichostatin A Protocadherin 9 Gastric carcinoma SGC-7901 cell Western blotting Human
作者简介 程琦(1985—),女(汉族),河南省周口市人,硕士,讲师;通讯作者:徐宛玲,E-mail:872189991@qq.com,Tel:(0395)2964510
  • 相关文献

参考文献1

二级参考文献14

  • 1彭胜祖,闵家新,蒋力.巨噬细胞集落刺激因子对肺癌细胞株A549在体外的抑制作用[J].第三军医大学学报,2007,29(5):419-421. 被引量:1
  • 2陈达凡,李建英,郑伟达,王小众.ERK信号通路与肝纤维化[J].国际消化病杂志,2007,27(5):370-372. 被引量:5
  • 3Phan SH. The myofibroblast in pulmonary fibrosis [ J]. CHEST, 2002,122(6) :286S-289S. 被引量:1
  • 4Kalluri R. EMT: when epithelial cells decide to become mescnchymal-like cells[ J ]. J Clin Invest, 2009,119 ( 6 ) : 1417- 1419. 被引量:1
  • 5Margadant C, Sonnenberg A. cancer and wound healing[ J ] Integrin-TGF-13 crosstalk in fibrosis EMBO Rep, 2010,11(2) :97-105. 被引量:1
  • 6Lin H, Lee E, Hestir K, et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome[J]. Science, 2008,320 ( 5877 ) : 807-811. 被引量:1
  • 7Willis BC, Borok Z. TGF-β-indueed EMT: mechanisms and implications for fibrotic lung disease [ J ]. Am J Physiol Lung Cell Mol Physiol, 2007,293 ( 3 ) : L525-L534. 被引量:1
  • 8Hwang SJ, Choi B, Kang SS, et al. Interleukin-34 produced by human fihroblast-like synovial cells in rheumatoid arthritis supports osteoclastogenesis [ J ]. Arthritis Res Ther, 2012,14 ( 1 ) : 1-10. 被引量:1
  • 9Ma D, Doi Y, Jin S, et al. TGF-β induced by interleukin-34-stimulated mieroglia regulates mieroglial proliferation and attenuates oligomeric amyloid β neurotoxicity [ J]. Neurosci Lett, 2012,529 (1) :86-91. 被引量:1
  • 10Stanley ER, Berg KL, Einstein DB, et al. Biology and action of colony-stimulating factor-1 [ J]. Mol Reprod Dev, 1997,46 ( 1 ) :4- 10. 被引量:1

共引文献1

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏