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良、恶性葡萄胎组织中P-选择素和HPA的表达及其临床意义 预览

Expression and clinical significance of P-selectin and HPA in benign and malignant hydatidform mole tissues
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摘要 目的 探讨P-选择素和乙酰肝素酶(heparanase,HPA)在良、恶性葡萄胎组织中的表达特点及临床意义。方法 采用免疫组化SP法及RT-PCR技术检测40例良性葡萄胎、12例恶性葡萄胎组织中P-选择素和HPA的表达情况,同时对比观察40例正常胎盘绒毛。结果 P-选择素和HPA在正常胎盘绒毛、良性葡萄胎、恶性葡萄胎组织中蛋白的阳性表达率、阳性表达强度及mRNA相对表达量均呈逐渐上升趋势,组间比较差异均有统计学意义(P均<0. 05)。P-选择素和HPA在良、恶性葡萄胎中的表达均呈正相关(P均<0. 05)。结论 P-选择素和HPA过表达促进葡萄胎的发生、发展,检测两者表达对于良、恶性葡萄胎的鉴别诊断及预后评估有重要意义,有望成为葡萄胎恶性发展的生物学预测指标。 Purpose To investigate the expression characteristics and significance of P-selectin and HPA in benign and malignant hydatidform mole tissues.Methods By using immunohistochemical technique SP and RT-PCR,the expression of Pselectin and HPA was detected in 40 cases of benign hydatidiform mole tissues,and 12 malignant hydatidiform mole tissues.Meanwhile,40 cases of normal placental villi tissues were observed.Results The protein positive rate and protein relative quantity expression and the mRNA relative quantity expression of P-selectin and HPA were gradually increasing in normal placenta villi tissues,benign hydatidiform mole tissues,and malignant hydatidiform mole tissues.The differences between groups were statistically significant(all P<0.05).The expression of P-selectin and HPA showed positive correlation(all P<0.05)in benign and malignant hydatidform mole tissues.Conclusion The over-expression of P-selectin and HPA promotes the occurrence and development of hydatidform mole.The detection of the expression levels of P-selectin and HPA is of great significance for the differential diagnosis and prognosis assessment of benign and malignant hydatidform mole.It could become a biological predictor of malignant development of hydatidform mole.
作者 齐洁敏 于淑莉 白兴武 王闯 QI Jie-min;YU Shu-li;BAI Xing-wu;WANG Chuang(Department of Pathology,Chengde Medical University,Chengde 067000,China;Department of Pathology,Chengde City Maternal and Child Health Hospital,Chengde 067000,China;Department of Internal Medicine,Chengde Nursing Vocational College,Chengde 067000,China)
出处 《临床与实验病理学杂志》 CAS CSCD 北大核心 2019年第3期273-277,共5页 Chinese Journal of Clinical and Experimental Pathology
基金 河北省科技厅项目(15277703D) 河北省高校重点学科建设项目[冀教高(2013)4号].
关键词 葡萄胎 P-选择素 HPA 免疫组织化学 RT-PCR hydatidform mole P-selectin HPA immunohistochemistry RT-PCR
作者简介 齐洁敏,女,硕士生导师,教授。E-mail:qijiemin@126.com
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