期刊文献+

类风湿性关节炎患者关节滑膜液浸润的T细胞表达特性 预览 被引量:4

The characteristics of synovium fluid from patients with Rheumatoid Arthritis
在线阅读 下载PDF
分享 导出
摘要 目的:为研究类风湿性关节炎(RA)患者关节滑膜液浸润的淋巴细胞介导自身免疫病的特性,分析了22例RA患者滑膜液中淋巴细胞的免疫表型、对II型胶原的反应频率及IL-10、IL-12的分泌格局.方法:用流式细胞术分别测定滑膜液和外周血淋巴细胞表型,并采用国际标准半有限稀释法分析了关节滑膜液中浸润的淋巴细胞对II型胶原(CII)的反应频率,同时用ELISA方法检测了滑膜液与外周血中IL-10与IL-12含量.结果:滑膜液中的T淋巴细胞的表型分别为CD4(39.6%±10.5%)和CD8细胞(36.4%±16.4%),CD4/CD8细胞比值显著低于外周血,且同时表达CD16和CD56的活化NK细胞占15.5%±11.1%.T细胞受体谱取用表明仍以αβTCR为主(69.6%±15.7%).有意义的是:滑膜液中的T细胞对CII的反应频率为15.2×10-6,远远高于外周血(4.0×10-6).IL-12含量为(419.9±89.2)pg/ml,IL-10含量为(187.7±34.5)pg/ml,与外周血中这2种细胞因子的含量[分别为IL-12:(65.32±34.2)pg/ml和IL-10:(85±12.7)pg/ml]比较,具有显著的统计学差异.结论:上述实验结果表明这种具有表达特性的浸润T细胞介导了RA患者关节滑膜组织的免疫损伤. Objective:To explore the mechanism of the Rheumatoid Arthritis(RA) mediated by autoreactive T lymphocytes infiltrated in the synovium fluid of patients with RA.Methods:Samples from 22 patients studied in the report.The phenotypy of the lymphocytes infiltrated in the synovium fluid was detected by immunefluorsence using flow cytometer.The frequency of human collagen II specific T cells existed in the synovium fluid of RA patients was analyzed using a standard split well method.The cytokine profile was determinded by ELISA.Results:Majority of the T cell subsets infiltrated in the synovium were demonstrated CD4 and CD8 positive cells in which the percent of the CD4 positive was 39.6%±10.5% and CD8 T cells were 36.4%±16.4% respectively.The ratio that CD4 divided CD8 was much lower than that of found in the PBL of the RA patients.Majority of them used αβTCR(69.6%±25.7%).There was also a small popular of activated NK with CD16 and CD56 positive (15.5%±11.5%) .The frequency of the collagen II specific T cells in synovium fluid was 12.5×10 -6 compared with this the collagen II specific T cells in the PBL from same patients showed little lower at the range of 4×10 -6 .The concentration of IL 12 in the synovium fluid was very high 〔(419.9±89.2)pg/ml〕 and IL 10 was relative lower〔(187.7±34.5)pg/ml〕.In contrast,the level of both the cytokine in the serum from same patients were very lower,in which the IL 12 gave (65.32±34.2)pg/ml and IL 10 showed (85±12.7)pg/ml respectively.Conclusion:The data here reported indicated that both lymphocyte infiltrated and cytokines involved the lesion of the joint tissues in the RA patients.
作者 张冬青 李宁丽 王利 余奇文 沈佰华 臧敬五 冯国璋 许荣 倪立清 ZHANG Dong Qing,LI Ning Li,WANG Li et al.Shanghai Immunology Institute,Shanghai Second Medical University,Shanghai 200025,China FENG Guo Zhang,XU Rong,NI Li Qing.Shanghai Guanghua Hospital,Shanghai 200052,China
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2003年第4期 275-277,共3页 Chinese Journal of Immunology
基金 上海市优秀学科带头人项目,国家自然科学基金,上海市科委资助项目
关键词 类风湿性关节炎 关节滑膜液 浸润 T细胞 Rheumatoid arthritis(RA) Synovium fluid Collagen II Cytokine profile
  • 相关文献

参考文献1

  • 1李宁丽 张冬青 周光炎.从健康人外周血分离G1特异性淋巴细胞株[J].上海免疫学杂志,2000,16(1):17-17. 被引量:1

同被引文献22

  • 1杜延顺,黄秉仁.G蛋白信号调节因子的结构分类和功能[J].生理科学进展,2005,36(3):215-219. 被引量:5
  • 2李宁丽 路丽明 张冬青 等.人可溶性TRAIL分子表达与生物学活性研究[J].上海第二医科大学学报,2003,23:10-10. 被引量:1
  • 3路丽明 李宁丽 张冬青 等.人TRAIL胞外段表达与生物学活性研究[J].中国实验诊断学杂志,2003,7:477-477. 被引量:1
  • 4Wilske KR,Yocum DE.Rheumatoid arthritis:The status and future of combination therapy[J].J Rheumatol,1996,23(Suppl 44):1. 被引量:1
  • 5Golub TR,Sonim DK,Tamayo P,et al.Molecular classification of cancer:class discovery and class prediction by gene expression monitoring[J].Science,1999,286(5439):531-537. 被引量:1
  • 6Becker N,Nieters A,Rittgen W.Single nucleotide polymorphism-disease relationships:statistical issues for the performance of association studies[J].Mutat Res,2003,525:11-18. 被引量:1
  • 7Jin N,Rafat A,Denis E,et al.Communication between tyrosine kinase pathway and myosin light chain kinase pathway in smooth muscle[J].American Physiology,1996,271(4part2):H1348. 被引量:1
  • 8Becker N, Nieters A, Rittgen W. Single nucleotide polymorphism-disease relationships: statistical issues for the performance of association studies. Mutat Res, 2003, 525:11-18. 被引量:1
  • 9Wagner U, Schulze-Koops H. T cells-do they control rheumatic immune responses? Z Rheumaol, 2005, 64: 377-382. 被引量:1
  • 10Toubi E, Kessel A, Mahmudov Z, et al. Increased spontaneous apoptosis of CD4^+CD25^+T cells in patients with active rheumatoid arthritis is reduced by infliximab. Ann N Y Acad Sci, 2005,1051: 506-514. 被引量:1

引证文献4

二级引证文献25

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈