期刊文献+

bFGF基因修饰的间充质干细胞/多孔β—TCP陶瓷复合移植修复兔节段性骨缺损 预览 被引量:15

MOLECULAR TISSUE ENGINEERING: APPLICATIONS FOR THE REPAIRING OF SEGMENTAL BONE DEFECTS USING POROUS β-TCP CERAMICS AND MESENCHYMAL STEM CELLS TRANSDUCED WITH THE bFGF GENE
在线阅读 下载PDF
收藏 分享 导出
摘要 目的:探讨碱性成纤维细胞生长因子(bFGF)基因转染间充质干细胞(MSCs)能否构建出具有良好生物学活性的组织工程化人工骨,提高骨缺损修复质量.方法:体外将具有促进MSCs增殖分化及刺激毛细血管增殖等多重生物学效应的bFGF基因转入骨组织工程首选种子细胞MSCs,并与具有良好力学强度及生物活性的可降解β磷酸三钙(β-TCP)多孔基质材料复合移植,修复同种异体兔桡骨15mm长节段性骨缺损.以单纯空载体转染MSCs为对照,通过扫描电镜、X线、组织学及免疫组化等方法检测.结果:转基因细胞/基质材料体外复合培养扫描电镜观察证实bFGF基因修饰的MSCs增殖分化活性明显优于对照组.体内实验X线及组织学检测结果显示实验组骨缺损正常修复,有大量活跃的新骨及丰富的毛细血管生成;对照组各时间点则以纤维结缔组织为主,未见明显的新骨及血管生成.免疫组化结果显示转基因细胞可持续表达bFGF至少4周以上.结论:把组织工程学与分子生物学有机结合,使bFGF持续高效发挥作用,研制出具有良好生物学活性的组织工程化人工骨,为高质量地修复骨缺损,特别是治疗骨折不愈合、股骨头缺血性坏死等疾病奠定了良好的基础. Objective: Basic fibroblast growth factor is one of the most prominent osteogenic growth factors that have the potential to accelerate bone healing by promoting the proliferation and differentiation of mesenchymal stem cells(MSCs) and the regeneration of capillary blood vessels. However, the short biologic half lives of growth factors may impose severe restraints on their clinical usefulness. Gene based delivery systems not only provide a better way of achieving a sustained high concentration of growth factors locally in the defect, but also could deliver their products in a more biologically active form than that achieved by exogenous application of recombinant proteins. The objective of this experimental study is to in vestig ate whether the bFGF gene modified MSCs could enhance the repair of large segmental bone defects. Methods: The pcDNA3-bFGF gene transduced periosteum-derived MSCs were seeded into biodegradable porous β tricalcium phosphate (β-TCP) ceramics and allografted into the 15mm critical-sized segmental bone defects in the radius of 18 New Zealand white rabbits. The pure pcDNA3 vector gene transduced MSCs were taken as a control. The follow-up times were 2,4,6,8,10 and 12 weeks. Scanning electron microscopic, roentgenographic, histological and immunohistological studies were performed. Results: In vivo, the proliferation and differentiation of bFGF gene transduced MSCs were more active than that of the control groups. Significantly more active new bone and capillary blood vessels were regenerated in pores of the ceramics loaded with bFGF gene transduced MSCs than that in the control groups. Intraosseous gene expression lasted at least 4 weeks. Conclusion: Transfer of gene encoding bFGF to MSCs increases its osteogenesis property and provides rich blood supply for the bone repairing. This molecular tissue engineering approach combining the principles of tissue engineering with principles of molecular biology, could be of potential benefit to accelerate bone healing, especially those caused by atro
作者 郑启新 郭晓东 刘勇 段德宇 吴永超 ZHENG Qi xin, GUO Xiao dong, LIU Yong, DUAN De yu, WU Yong chao(Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022)
出处 《中国生物医学工程学报》 EI CAS CSCD 北大核心 2003年第2期 171-177,共7页 Chinese Journal of Biomedical Engineering
基金 国家自然科学基金,国家自然科学基金
关键词 BFGF基因修饰 间充质干细胞 多孔β-TCP陶瓷 复合移植 修复 兔节段性骨缺损 Blood vessels Bone Cells Ceramic materials Porous materials Scanning electron microscopy
  • 相关文献

参考文献17

二级参考文献14

  • 1Zheng Q X,J Tongji Med Univ,1992年,12卷,173页 被引量:1
  • 2Kim T,Trans plant Proc,1997年,29卷,858页 被引量:1
  • 3Qin L,Human Gene Therapy,1996年,7卷,1981页 被引量:1
  • 4Pash JM,Delany AM,Adamo ML et al. The Journal of Endocrinology . 1995 被引量:1
  • 5Onyia JE,Libermann TA,Bidwell J et al. Journal of Cellular Biochemistry . 1997 被引量:1
  • 6Veenstra TD,Fahnestock,Kumar R et al. Biochemistry . 1998 被引量:1
  • 7Mohan S,Baylink DJ. Clinical Orthopaedics . 1991 被引量:1
  • 8Fang J,Zhu YY,Smiley E et al. Proceedings of the National Academy of Sciences of the United States of America . 1996 被引量:1
  • 9Gentry LE,Webb NR,Lim GJ et al. Molecular and Cellular Biology . 1987 被引量:1
  • 10Yoshikawa H,Shimizu K,Nakase T et al. Clinical Orthopaedics . 1994 被引量:1

共引文献29

同被引文献389

引证文献15

二级引证文献50

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏