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三角梅扦插枝化学诱变育种 预览

Chemical Mutagenesis for the Cuttings of Bougainvillea glabra‘Mrs.Eva’and B.×buttiana‘Miss Manila’
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摘要 三角梅是一种重要的热带和亚热带观赏植物。由于三角梅有性繁殖困难,诱变育种是目前比较可行的育种方式。本研究采用甲基磺酸乙酯(EMS)、叠氮化钠(NaN3)和平阳霉素(PYM)3种不同类型诱变剂对‘伊娃夫人’和‘马尼拉小姐’2个三角梅品种扦插枝进行了化学诱变。研究发现‘伊娃夫人’和‘马尼拉小姐’20 h EMS半致死剂量分别为0.22%和0.62%。随EMS浓度增加,2个品种扦插枝发芽数和最长分枝长呈下降趋势,叶绿素和类胡萝卜素含量逐渐增加;‘伊娃夫人’叶片逐渐向暗色调变化,‘马尼拉小姐’叶片向红色色调变化。20 h酸性NaN3处理下,‘伊娃夫人’无法存活,‘马尼拉小姐’成活率为82%~92%;随NaN3浓度升高,‘马尼拉小姐’发芽数呈显著下降趋势。相比对照,20 h PYM显著提高了2个三角梅品种扦插枝最大分枝长度,但对扦插枝存活率无显著影响。中高浓度EMS和PYM处理‘马尼拉小姐’三角梅出现了叶缘缺刻、叶表褶皱和双叶尖等变异株。 Bougainvillea Comm.ex Juss is an important ornamental plant in the tropical and sub-tropical regions of China.Because the sexual reproduction of Bougainvillea is difficult,mutation breeding is an available breeding method.The present study used ethylmethane sulfonate(EMS),sodium azide(NaN3)and pingyangmycin(PYM)to mutagenize the cuttings of B.glabra‘Mrs.Eva’and B.×buttiana‘Miss Manila’,respectively.In 20 h EMS treatments,the half lethal dose of‘Mrs.Eva’and‘Miss Manila’cuttings was 0.22%and 0.62%,respectively.With the increasing of EMS concentration,the shoot number and length of the longest branch of two Bougainvillea varieties showed a decreasing trend,while the chlorophyll and carotenoid content increased gradually.With the increasing of EMS concentration,the leaf hue of‘Mrs.Eva’changed to grayish tone and that of‘Miss Manila’changed to red tone gradually.Almost all‘Mrs.Eva’cuttings died in 20 h NaN3 treatments,while the survival rate of‘Miss Manila’cuttings was 82%-92%.With the increasing of NaN3 concentration,the shoot number of‘Miss Manila’decreased significantly.20 h PYM treatments significantly improved the length of the longest branch of two Bougainvillea varieties in contrast to the control,but had no significant influence on the survival rate.Moreover,variant seedlings were found in EMS and PYM treatments,representing transformative leaf margin,wrinkled leaf surface or double leaf tips.
作者 常圣鑫 黄素荣 徐世松 杨光穗 CHANG Shengxin;HUANG Surong;XU Shisong;YANG Guangsui(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture and Rural Affairs,Danzhou,Hainan 571737,China;Hainan Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization,Danzhou,Hainan 571737,China)
出处 《热带作物学报》 CSCD 北大核心 2019年第2期238-246,共9页 Chinese Journal of Tropical Crops
基金 中央级基本科研业务费项目“三角梅四倍体诱变研究’(No.1630032017090) 海南省2018年重大科技项目“三角梅盆栽关键技术研究与示范”。
关键词 三角梅 甲基磺酸乙酯 叠氮化钠 平阳霉素 Bougainvillea ethylmethane sulfonate sodium azide pingyangmycin
作者简介 常圣鑫(1987—),男,博士,助理研究员,研究方向:热带花卉种质资源;通信作者(Corresponding author):杨光穗(YANG Guangsui),E-mail:13976572870@163.com。
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