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诺如病毒GⅡ.13P蛋白的糖结合特征

Glycan binding pattern of norovirus GⅡ.13P protein
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摘要 目的分析诺如病毒(norovirus,NoV)GⅡ.13毒株09N3145与组织血型抗原(histo-blood groups antigens,HBGAs)的结合特性。方法表达并纯化GⅡ.13 09N3145的P区蛋白,通过唾液及寡糖结合试验确定其与多种不同寡糖的结合特征,并以GⅡ.13/2010 P区蛋白与Lea寡糖复合物结构为模板,对GⅡ.13 09N3145 P蛋白进行同源模拟。结果 GⅡ.13 09N3145的P区蛋白可以与A、B、O+和非分泌型O-发生结合;可以与Lec双糖抗原和乳糖发生结合,而与A、B、H、Lewis型等寡糖无明显结合。分子模拟显示P蛋白使用一个可能的结合位点与半乳糖环结合。结论确定了GⅡ.13 09N3145 P蛋白与HBGAs的结合特征,为NoV宿主相互作用、人诺如病毒(human norovirus,huNoV)进化、流行以及天然抗病毒策略的提出奠定基础。 Objective To analyze the binding pattern between the P protein of GⅡ.13 strain(09 N3145)and histo-blood group antigens(HBGAs).Methods The P protein of GⅡ.13 09 N3145 was expressed and purified,of which the binding feature to various glycans was evaluated by saliva and glycan binding assays.A homology modeling of GⅡ.1309 N3145 P protein was built based on the complex structure of P protein of GⅡ.13/2010-Lea.Results GⅡ.1309 N3145 P protein showed binding to the types A,B and O+secretor as well as O-non-secretor saliva.However,the P protein showed binding to Lec disaccharide antigens and lactose,while no significant binding to oligosaccharides of types A,B,H and Lewis.The homology modeling indicated that P protein bound to galactose ring using a potential glycan binding site.Conclusion The binding pattern between P protein of GⅡ.13 09 N3145 and HBGAs was determi-ned.These findings provided a basis for elucidating the mechanism of evolution,epidemiology and natural antiviral strategy of human norovirus(huNoV).
作者 丛鑫 李涵博 王鹏飞 靳淼 李丹地 段招军 CONG Xin;LI Han-bo;WANG Peng-fei;JIN Miao;LI Dan-di;DUAN Zhao-jun(National Institute for Viral Disease Control and Prevention,Chinese Center for Diseases Control and Prevention,Beijing 102206,China)
出处 《中国生物制品学杂志》 CAS CSCD 2019年第3期261-264,共4页 Chinese Journal of Bilogicals
基金 国家科技重大专项(2017ZX10104001-003-001 2018ZX10-711-001) 国家自然科学基金面上项目(81472003).
关键词 诺如病毒 受体结合 GⅡ.13P蛋白 同源模拟 Norovirus Receptor binding GⅡ.13P protein Homology modeling
作者简介 通讯作者:段招军,E-mail:zhaojund@126.com.
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