期刊文献+

淋巴因子对人PBMNC的LAK细胞形成、增殖和细胞毒作用的影响 预览 被引量:1

EFFECTS OF LYMPHOKINES ON GENERATION,PROLIFERATION AND CYTOTOXICITY OF LAK CELLS FROM PBMNC OF HEALTHY ADULTS
在线阅读 下载PDF
分享 导出
摘要 应用乳酸脱氢酶释放法对重组白介素2,粗制天热白介素2,重组肿瘤坏死因子和天热免疫活性肽等淋巴因子诱导人PBMNC为LAK细胞以及LAK细胞对靶细胞(人外周血淋巴细胞,人食管癌109细胞株和人红白血病细胁株K602)的红细胞毒作用分别于培养的第4d和第7d进行了检测。结果显示:①四个配伍组和一个重组白介素2组的多克隆LAK细胞攻击溶解109缅滟株和K602细胞株的水平被动于28%和66%之间,中位数为47%,而对正常人外周血淋巴细胞均无细胞毒作用;②重组白介素2和粗制天然白介素2配伍培养LAK细胞的增殖协力约高于重组白介素2和重组肿瘤坏死因子组,以及重组白介素2和免疫活性肽组增殖协力的8倍,③在诱导LAK细胞的过程中其增殖量和其细胞毒活性可以不同步。建议临床土治疗晚期肿瘤病人除用LAK细胞和重组白介素2外,首先选用天然白介素2。 In this paper,rIL- 2, impure nlL- 2, rTNF and IAP were used to induce transforming of PBMNC cultured in RPMI media into LAK cells and the cytotoxicity or LAK cells against target cells(human allogeneic peripheral blood lymphocyte-PBL, human esophageal cancer 109 cell line-EC109 and human erythroleukemia cell line- K_(562) was measured by a LDH release assay at day 4 and 7.The results showed that(1) the polyclonal LAK cells in each of 4 co-culture media and one medium with rIL-2 alone lysed EC109 and K_(562) at levels varied between 28%and 66% ith a medi-an of 47%but possessed no the activity of lysing normal human periph-eral blood lymphocytes;(2)the synergism in proliferating of LAK cells in medium supplemented with rIL-2 and impure nIL-2 was approximately 3 times that caused by the additive effect of rIL-2 with either rTNF or IAP;(3)the proliferation and cytotoxicity of LAK cells might not be synchro-nous in the induction course.It is therefore suggested from the data sum-marized above that the impure nIL-2 be selected clinically and preferentially, in addition to LAK cells and rIL-2,to cure the patients with advanced cancer.
作者 戴寿芝 周悌 杨志伟 阚捷 王慕娣 裴秀英 韩梅 高乐 胡忠义 Dai Shouzhi,Yang Zhiwei Kan Jie,et al(Dept.of Immunology,Ningxia Med. Coll.)Zhou Ti(Dept.of Surgery,Affiliated Hospital, Ningxia Med. Coll.)
出处 《宁夏医学院学报》 1995年第1期 1-5,共5页 Journal of Ningxia Medical College
关键词 淋巴因子 人PBMNC LAK细胞 细胞形成 细胞增殖 细胞毒性 肿瘤 生物疗法 白细胞介素2 肿瘤坏死因子 lymphokine interleukin -2 tumor necrosis factor immuno-active peptide lymphokine-activated killer cell
  • 相关文献

参考文献2

同被引文献2

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈