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紫草的蒙医传统奶炮制工艺及质量标准研究

Traditional Mongolian Medicine Milk Processing Method and Quality Standard Study on Arnebia euchroma(Royle)Johnst
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摘要 目的:采用传统的蒙药炮制方法,对紫草药材进行奶炮制,通过HPLC等对其生品及其炮制品的有效成分进行含量测定,为紫草的奶炮制品的质量标准控制提供依据。方法:通过TLC进行理化鉴别,UV法测定其生品和炮制品的羟基萘醌总色素的含量,HPLC法测定其β,β’-二甲基丙烯酰阿卡宁的含量。结果:TLC结果表明,紫草的炮制品和对照药材的斑点一致,表明奶炮制后其总色素成分基本没有发生变化;UV和HPLC的结果表明,炮制品的羟基萘醌总色素(0.56%~2.5%)和β,β’-二甲基丙烯酰阿卡宁(0.020%~0.52%)的含量与生品比较均有不同程度下降。结论:本研究可为今后的蒙医药中紫草的奶炮制品的质量标准及药理实验提供实验依据。 Objective:According to traditional Mongolian medicine processing methods,Arnebia euchroma(Royle)Johnst was processed by milk and the active ingredients of the raw and its processed products are determined by HPLC,which provides a basis for the quality standards of the milk products of Arnebia euchroma(Royle)Johnst.Methods:Physical and chemical identification was carried out by TLC.The total content of hydroxynaphthoquinones in raw and processed products was determined by UV method,and the content ofβ,β’-dimethylacryloaikanin was determined by HPLC.Results:TLC results showed that the spots of the processed products of Arnebia euchroma(Royle)Johnst were the same as those of the control,indicating that the total pigment components of the processed products did not change after being processed.The results of UV and HPLC showed that the contents of hydroxynaphthoquinones(0.56%~2.5%)andβ,β’-dimethylacryloaikanin(0.020%~0.52%)in processed products decreased compared with that of raw materials.Conclusion:This study provides experimental evidence for the quality standards and pharmacological experiments of Arnebia euchroma(Royle)Johnst in future Mongolian medicine.
作者 苏日娜 牧丹 那生桑 Surina;Mudan;Nasensang(Inner Mongolia Medical University,Hohhot 010110,China)
机构地区 内蒙古医科大学
出处 《亚太传统医药》 2019年第2期64-67,共4页 Asia-Pacific Traditional Medicine
基金 内蒙古自治区政府重大项目(MB 2017-01).
关键词 紫草 奶炮制 HPLC β β’-二甲基丙烯酰阿卡宁 Arnebia euchroma(Royle)Johnst Milk Process HPLC β,β’-dimethylacryloaikanin
作者简介 苏日娜(1983-),女,博士,内蒙古医科大学讲师,研究方向为药物分析;通讯作者:那生桑(1959-),男,博士,内蒙古医科大学教授,研究方向为蒙药化学。E-mail:nasang56@sina.com
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