目的探讨凋亡抑制基因survivin在卵巢上皮性癌中的表达,及其与bcl-2、bax蛋白表达的相关性.方法应用逆转录聚合酶链反应(RT-PCR)技术,检测35例卵巢上皮性癌组织中survivin 基因的表达;应用免疫组织化学链霉亲和素-生物素-过氧化物酶复合物(SABC)方法,检测bcl-2及bax蛋白的表达,并与卵巢上皮性交界性肿瘤、良性卵巢肿瘤及正常卵巢组织各10例进行对照.结果卵巢上皮性癌、交界性肿瘤组织中,survivin 基因的表达率分别为83%及80%,显著高于良性卵巢肿瘤及正常卵巢组织的表达率20%及0%( P均<0.05).survivin基因表达与卵巢上皮性癌的临床分期、病理学类型、组织学分级及淋巴结转移无相关性(P均>0.05).survivin基因表达与bcl-2蛋白表达呈正相关(P<0.01),而与bax蛋白表达呈负相关(P<0.05).结论 survivin基因可通过抑制癌细胞凋亡,对卵巢上皮性癌的发生和发展起作用;survivin基因可能与凋亡相关基因bcl-2、bax,在卵巢上皮性癌的发展中分别起协同和拮抗作用.
Objective To study the expression of apoptosis related gene survivin and its relation with expression of bcl-2, bax protein in epithelial ovarian cancer. Methods Expression of survivin gene was evaluated by reverse transcription polymerase chain reaction (RT-PCR) in 35 cases of epithelial ovarian cancer,and compared with that in 10 cases of borderline cancer,10 cases of benign tumors and 10 cases of normal tissue. Expression of bcl-2 and bax was detected by immunohistochemistry streptomycin-avidin-biotin-peroxidase complex (SABC) method, and correlation between them was analyzed. Results Expression of survivin gene was detected in a significantly greater proportion in epithelial ovarian cancer and borderline tumors than benign tumors and normal tissue. There was no relationship between survivin gene expression and FIGO stage, histologic grade, pathological type and lymphatic metastasis. Expression of bcl-2 and bax protein was positively and negatively correlated with expression of survivin gene respectively. Conclusion survivin gene may play an important role in pathogenesis of ovarian cancer, apoptosis related gene bcl-2 may have a synergic role and bax have an antagonistic role with survivin gene in formation and progression of ovarian cancer.
Chinese Journal of Obstetrics and Gynecology