[目的]探讨自发性高血压大鼠(SHR)颈动脉血管间质胶原重构的特点及应用厄贝沙坦逆转的意义.[方法]选择16周龄雄性SHR 24只分为SHR组、厄贝沙坦组(30 mg*kg-1*d-1),另选同龄雄性Wistar大鼠12只为正常对照组;颈总动脉切片采用HE染色、苦味酸-天狼星红(PSR)染色分别检测颈动脉壁厚/腔径比值(W/C)、胶原面积百分比(VFC).[结果]SHR组W/C值较正常对照组高(P<0.01),厄贝沙坦组治疗20周后,较SHR组下降(P<0.01);SHR组胶原面积百分比较正常对照组高(P<0.01),厄贝沙坦组治疗20周后,较SHR组也有下降(P<0.05);SHR组血管壁厚/腔径比值和胶原面积百分比均与SBP相关(均P<0.05);厄贝沙坦组血管壁厚/腔径比值与SBP相关(P<0.05),而胶原面积百分比则与血压的相关性不明显.[结论]SHR颈动脉血管存在以壁厚/腔径比值和胶原面积百分比增高为特点的重构,应用厄贝沙坦时血管壁厚/腔径比值能逆转,但血管胶原面积百分比只有一定程度的逆转.
To investigate the characteristics of vascular collagen remodeling of the carotid artery and significance of irbesartan to reverse the effect in spontaneous hypertension rats (SHRs).Twenty-four male SHRs (age 16 wks) were selected and divided into 2 groups:①SHR group,12 SHRs; ②Irbesartan group, 12 SHRs were treated with irbesartan in a dosage of 30 mg·kg -1 ·d -1 . In addition, 12 male Wistar rats with the same age served as ③ Normotensive control group. The vascular volume fraction of collagen (VFC) and the ratio of carotid artery wall thickness /cavity diameter (W / C) were detected by picrosirius red and HE staining, respectively.In SHR group, VFC and W / C were significantly higher than those of controls (both P<0.01); these two indexes were correlated to their systolic blood pressure(SBP)(P<0.05).Twenty wks after treatment, VFC and W/C in irbesartan group decreased obviously (P<0.01) and considerably(P<0.05) as compared with those in SHR group; but correlation of the former to blood pressure was not so prominent, while the latter was correlated with SBP(P<0.05).[Conclusion]A remodeling with characteristics of increased VFC and W/C exists in the carotid artery of SHR. Irbesartan is beneficial to reverse the W/C ratio of carotid artery in SHR , but it can only reverse their VFC to certain extent.
Journal of Clinical Research