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The Use of RIDA<sup>®</sup>COUNT for Monitoring the American Foulbrood Pathogen 预览
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作者 Stepan Ryba Vaclav Kristufek Dalibor Titera 《兽医学(英文)》 2012年第4期233-236,共4页
American Foulbrood (AFB) is currently one of the most dangerous diseases in honeybees due to its high virulence and worldwide spread. Quick evaluation of the diagnosis of this disease is crucial. Successful eradicatio... American Foulbrood (AFB) is currently one of the most dangerous diseases in honeybees due to its high virulence and worldwide spread. Quick evaluation of the diagnosis of this disease is crucial. Successful eradication in the area indicates a need to test all bee colonies, but this is expensive and time consuming. A new method of detecting Paenibacillus larvae using the RIDA?COUNT test (R-Biopharm AG, Germany) was verified in the present study. The test is based on the principle of the cultivation test with MYPGPn medium, coloration of the bacteria with TTC (2,3,5-triphenyltetra-zoliumchloride) chromophore, and heat treatment of the sample. Using this new method, color-highlighted colonies of P. larvae can be established on the seventh day after inoculating the spores. An identical number of colonies grown with the classic cultivation test on Petri dishes containing MYPGPn medium or RIDA?COUNT-P. larvae (RC-PL) sheets were verified. 展开更多
关键词 AFB APIS MELLIFERA L. Bacterial Disease Diagnosis Diseases of HONEYBEES MYPGPn Medium PAENIBACILLUS larvae
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