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Expressing an (E)-β-farnesene synthase in the chloroplast of tobacco affects the preference of green peach aphid and its parasitoid
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作者 Gen-Ping Wang Xiu-Dao Yu +2 位作者 Jia Fan Cheng-She Wang Lan-Qin Xia 《植物学报:英文版》 SCIE CAS CSCD 2015年第9期770-782,共13页
(E)-β-Farnesene (EbF) synthase catalyses the production of EbF, which for many aphids is the main or only component of the alarm pheromone causing the repellence of aphids and also functions as a kairomone for ap... (E)-β-Farnesene (EbF) synthase catalyses the production of EbF, which for many aphids is the main or only component of the alarm pheromone causing the repellence of aphids and also functions as a kairomone for aphids’ natural enemies. Many plants possess EbF synthase genes and can release EbF to repel aphids. In order to effectively recruit the plant-derived EbF synthase genes for aphid control, by using chloroplast transit peptide (CTP) of the small subunit of Rubisco (rbcS) from wheat (Triticum aestivum L.), we targeted AabFS1, an EbF synthase gene from sweet wormwood (Artemisia annua L.), to the chloroplast of tobacco to generate CTP + AaβFS1 transgenic lines. The CTP + AaβFS1 transgenic tobacco plants could emit EbF at a level up to 19.25 ng/day per g fresh tissues, 4–12 fold higher than the AabFS1 transgenic lines without chloroplast targeting. Furthermore, aphid/parasitoid behavioral bio-assays demonstrated that the CTP +AaβFS1 transgenic tobacco showed enhanced repellence to green peach aphid (Myzus persicae) and attracted response of its parasitoid Diaeretiella rapae, thus affecting aphid infestation at two trophic levels. These data suggest that the chloroplast is an ideal subcellular compartment for metabolic engineering of plant-derived EbF synthase genes to generate a novel type of transgenic plant emitting an alarm pheromone for aphid control. 展开更多
关键词 Aphid CHLOROPLAST Oiaeretiella rapae (E)-β-farnesene SYNTHASE MYZUS persicae TOBACCO
Metabolic Engineering of Plant-derived (E)-β-farnesene Synthase Genes for a Novel Type of Aphid-resistant Genetically Modified Crop Plants 被引量:3
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作者 Xiu-Dao Yu John Pickett +4 位作者 You-Zhi Ma Toby Bruce Johnathan Napier Huw D. Jones Lan-Qin Xia 《植物学报:英文版》 SCIE CAS CSCD 2012年第5期 282-299,共18页
蚜虫是每年引起重要产量庄稼植物的损失的主要农业害虫。因为杀虫药剂抵抗,非目标有机体上的潜在的否定效果和环境污染的开发,为长期的蚜虫控制的杀虫药剂上的过多的依赖是不受欢迎的。经由行动的一个无毒的模式为抵抗设计到蚜虫的转... 蚜虫是每年引起重要产量庄稼植物的损失的主要农业害虫。因为杀虫药剂抵抗,非目标有机体上的潜在的否定效果和环境污染的开发,为长期的蚜虫控制的杀虫药剂上的过多的依赖是不受欢迎的。经由行动的一个无毒的模式为抵抗设计到蚜虫的转基因的庄稼能是有效其他的策略。(E)-- Farnesene (EF ) synthases 催化 EF 的形成,它为许多害虫蚜虫是在这些以内涉及化学通讯的警报 pheromone 的主要部件种类。EF 能被某些植物也综合,但是然后通常与禁止的混合物被污染。能够综合并且射出 EF 的庄稼植物的工程能引起蚜虫并且也的排斥把 EF 用作 foraging 暗示的生来的敌人的吸引力,因此最小化蚜虫群袭。在这评论,主机植物上的蚜虫的效果,对蚜虫 herbivory 的植物防卫和为在农业设置的蚜虫控制的生来的敌人的招募是简短介绍了。而且,导出植物的 EF synthase 基因克隆在经由遗传上修改的途径产生新奇蚜虫抵抗与他们的潜在的角色一起标明日期被讨论。 展开更多
关键词 蚜虫报警信息素 转基因作物 合成酶基因 代谢工程 植物源 农业害虫 环境污染 非靶标生物
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